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Merck

A6237

Sigma-Aldrich

Apyrase from potato

recombinant, expressed in Pichia pastoris, ATPase ≥1000 units/mg protein, lyophilized powder

Sinónimos:

Apyrase from potato, Adenosine 5′-diphosphatase, Adenosine 5′-triphosphatase

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100 UNITS
US$ 197,00
500 UNITS
US$ 720,00

US$ 197,00


Fecha estimada de envío13 de abril de 2025



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100 UNITS
US$ 197,00
500 UNITS
US$ 720,00

About This Item

Comisión internacional de enzimas:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54

US$ 197,00


Fecha estimada de envío13 de abril de 2025


recombinant

expressed in Pichia pastoris

Quality Level

form

lyophilized powder

ATPase activity

≥1000 units/mg protein

shipped in

wet ice

storage temp.

−20°C

General description

Apyrase, also known as ATP-diphosphohydrolase, is expressed in wide variety of plant and animal tissues. The commercially accessible apyrase enzyme is isolated from potato tubers.[1]

Biochem/physiol Actions

Apyrase hydrolyses the phosphoanhydride bonds of nucleoside tri- and di-phosphates in the presence of divalent cations.[1][2] It has wide substrate specificity for nucleotides. This property of the enzyme makes it suitable for different biotechnical applications, including DNA sequencing and platelet-aggregation inhibition.[1]

pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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Certificados de análisis (COA)

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Xuan Zhang et al.
European journal of medicinal chemistry, 199, 112397-112397 (2020-05-11)
Targeting BCL-XL via PROTACs is a promising strategy in reducing BCL-XL inhibition associated platelet toxicity. Recently, we reported potent BCL-XL PROTAC degraders that recruit VHL or CRBN E3 ligase. However, low protein expression or mutation of the responsible E3 ligase
Subin Mao et al.
Lab on a chip, 21(16), 3128-3136 (2021-06-29)
Integrins are key players in platelet adhesion and aggregation. Integrin molecular tensions, the forces transmitted by integrin molecules, are regulated by both mechanical and biochemical cues, and the outside-in and inside-out signaling has been extensively studied. While the mechanical properties
Methylotrophic yeast Pichia pastoris as a host for production of ATP-diphosphohydrolase (apyrase) from potato tubers (Solanum tuberosum).
Nourizad N, et al.
Protein Expression and Purification, 27(2), 229-237 (2003)
Cloning, sequencing, and expression of a human brain ecto-apyrase related to both the ecto-ATPases and CD39 ecto-apyrases1.
Smith T M & Kirley T L
Biochimica et Biophysica Acta, 1386(1), 65-78 (1998)
Celia Cordero-Sanchez et al.
Blood advances, 6(15), 4471-4484 (2022-06-14)
Store-operated Ca2+-entry is a cellular mechanism that governs the replenishment of intracellular stores of Ca2+ upon depletion caused by the opening of intracellular Ca2+-channels. Gain-of-function mutations of the 2 key proteins of store-operated Ca2+-entry, STIM1 and ORAI1, are associated with

Questions

  1. Hello. Is the concentration of 100 units in 1 mL of water? If not, how can I make the dilution to obtain 100 units? Thanks for you attention! Guilherme

    1 answer
    1. The solubility of this enzyme is tested on the finished product at 1 mL per vial in water. The 100-unit package size will be readily soluble in 1 mL, however, the number of actual units per vial should be reviewed in the lot-specific certificate of analysis. The vial content is typically greater than 100 units. The reconstitution volume would have to be adjusted accordingly in order to achieve a 100 unit per milliliter concentration.
      See the link below to review the sample Certificate of analysis:
      https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/A6237-100UN0000174833.pdf

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