A3188
Anti-Human IgG (γ-chain specific)−Alkaline Phosphatase antibody produced in goat
affinity isolated antibody, buffered aqueous glycerol solution
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About This Item
Número MDL:
Código UNSPSC:
12352203
Productos recomendados
origen biológico
goat
conjugado
alkaline phosphatase conjugate
forma del anticuerpo
affinity isolated antibody
tipo de anticuerpo
secondary antibodies
clon
polyclonal
Formulario
buffered aqueous glycerol solution
reactividad de especies
human
no debe reaccionar con
mouse
técnicas
direct ELISA: 1:30,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50
western blot: 1:30,000
Condiciones de envío
wet ice
temp. de almacenamiento
2-8°C
modificación del objetivo postraduccional
unmodified
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Descripción general
Human IgG antibodies are primarily responsible for the facilitation of humoral immune responses such as complement fixation, placental transport, and phagocytosis. Anti-human IgG (γ-chain specific) −alkaline phosphatase antibody can be used in immunohistochemistry and immunoelectrophoresis. It can also be used in western blotting. Goat anti-human IgG−alkaline phosphatase antibody reacts specifically with human IgG when tested against human IgA, IgG, IgM, Bence Jones κ, and λ myeloma proteins. The product is not reactive with mouse IgG or mouse serum proteins.
Inmunógeno
Purified human IgG.
Aplicación
Anti-human IgG (γ-chain specific) −alkaline phosphatase antibody can be used in immunocytochemical staining techniques. It can also be used for dot blot.
Proteins harvested from E. coli cultures were run on SDS-page gel for western blot analysis using alkaline phosphatase conjugated goat anti-human IgG (chain specific) at a concentration of 1:10000 for the secondary. Membranes were developed using BCIP/NBT substrate (Sigma).[1]
Proteins harvested from E. coli cultures were run on SDS-page gel for western blot analysis using alkaline phosphatase conjugated goat anti-human IgG (chain specific) at a concentration of 1:10000 for the secondary.[1] IgG was detected in human serum by Elisa using alkaline phosphatase conjugated goat anti-human IgG (gamma-chain specific) diluted 1:5000 in 0.25% BSA/PBS. Assay was developed using p-nitrophenyl phosphate substrate (1mg/ml Sigma).[2]
Otras notas
Antibody adsorbed with mouse serum proteins.
Forma física
Solution in 0.05 M Tris buffer, pH 8.0, containing 1 mM MgCl2, 10 mM glycine, 1% bovine serum albumin, 50% glycerol and 15 mM sodium azide as preservative
Cláusula de descargo de responsabilidad
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Código de clase de almacenamiento
10 - Combustible liquids
Clase de riesgo para el agua (WGK)
WGK 3
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Equipo de protección personal
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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