A2382
Azoalbumin
protease substrate
Sinónimos:
Sulfanilic acid-azoalbumin
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About This Item
Productos recomendados
biological source
bovine
Quality Level
form
powder
concentration
60-90% (Lowry)
technique(s)
tissue culture: suitable
solubility
0.01 M HCl: soluble 25 mg/mL
storage temp.
2-8°C
General description
Azoalbumin is a substrate for assaying proteolytic enzymes, majorly trypsin, pepsin and cysteine proteinase. The assay method offers precision and is easy to perform.
Application
Azoalbumin has been used:
- as a substrate for enzymatic assay of hyphal extracts
- for screening general protease activity in spent growth medium extract
- for screening S. rolfsii proteases
Preparation Note
Prepared from Bovine albumin, Fraction V (A 4503)
Substrates
A soluble chromogenic substrate for proteolytic enzymes.
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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B Cook et al.
Journal of reproduction and fertility, 51(1), 65-71 (1977-09-01)
No unusual steroid-binding proteins that might react with the oocyte or its investments could be detected in follicular fluid. Corticosteroid-binding globulin occurred in follicular fluid from pigs, sheep and cows, and sex hormone-binding globulin occurred in follicular fluid from sheep
Optimisation of cellobiose dehydrogenase production by the fungus Sclerotium (Athelia) rolfsii
Ludwig R and Haltrich D
Applied Microbiology and Biotechnology, 61(1), 32-39 (2003)
Inhibition of digestive trypsin-like proteases from larvae of several lepidopteran species by the diagnostic cysteine protease inhibitor E-64
Novillo C, et al.
Journal of Clinical Pathology, 27(3), 247-254 (1997)
Feces derived allergens of Tyrophagus putrescentiae reared on dried dog food and evidence of the strong nutritional interaction between the mite and Bacillus cereus producing protease bacillolysins and exo-chitinases
Erban T, et al.
Frontiers in Physiology, 7(3), 53-53 (2016)
A modified azoalbumin technique for the assay of proteolytic enzymes for use in blood group serology.
Phillips PK, et al.
Journal of Clinical Pathology, 37(3), 329-331 (1984)
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