602-05A

Hair Follicle Dermal Papilla Cells: HFDPC, adult

• Sinónimos: HFDPC, HFDPC cells
• NACRES: NA.81
• UNSPSC Code: 41106514
• Biological source: human scalp (hair follicle papilla)
• Growth mode: Adherent
• Morphology: mesenchymal
• Relevant disease(s): Alzheimer′s disease

Propiedades

• biological source: human scalp (hair follicle papilla)
• packaging: pkg of 500,000 cells
• manufacturer/tradename: Cell Applications, Inc
• growth mode: Adherent
• morphology: mesenchymal
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): Alzheimer′s disease
• shipped in: dry ice
• storage temp.: −196°C

Descripción

General description

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Human Hair Follicle Dermal Papilla Cells (HFDPC) are mesenchymal cells isolated from the hair papilla of normal human scalp hair follicles. Hair papilla, in the adult hair follicle, plays a crucial role in the dermal-epidermal interactions that control hair production and events of the hair growth cycle. HFDPC can therefore be used for studying cellular mechanisms organizing the hair follicle, and for the development and evaluation of hair growth products.

HFDPC have been used to demonstrate that IL-1α affects dermal papilla activity by decreasing androgen receptor expression and stimulating secretion of KFG, VEGF, IL-8 and GM-CSF (Boivin, 2006). They were also used to investigate how gene expression and cell proliferation are affected by testosterone analogues (Reiter, 2008), potential hair growth enhancing agents (Sasajima, 2008) and microbial metabolites (Takigawa, 2012). Using HFDPC, along with Human Epidermal Keratinocytes and Human Bronchial Epithelial Cells, scientists also studied mechanisms of cellular senescence, and develop ed methodology to extend cellular life span and immortalize the cells by inhibiting p16INK4a and introducing hTERT (Haga, 2007). Finally, HFDPC have been used to discover the link between the androgen-induced alopecia and circadian rhythms, mediated by a “clock gene” transcription factor BMAL1 (Watabe, 2013).

Application

dermatalogical interactions, hair production, induction and maintenance of hair growth, hair follicle organization, development and evaluation of hair growth products, effects and secretion of growth factors and cytokines, gene expression, cell proliferation, cell senescence, cell lifespan extension, in vitro screening of androgen blocking agents, reservoir of multi-potent stem cells, skin reconstruction, wound healing, signal transduction pathways, skin composites and substitutes, hair follicle neogenesis

Biochem/physiol Actions

Scalp

Preparation Note

• 2nd passage, >500,000 cells in Cell Basal Medium containing 20% FBS & 5% DMSO
• Can be cultured at least 6 doublings

Please refer to the HFDPC Culture Protocol.

Other Notes

Cell Basal Medium containing 20% FBS & 5% DMSO

Disclaimer

This product is regulated in France when intended for scientific purposes, including import, export, and conservation activities (Articles L.1211-1, paragraph 2, and L.1243-4 of the French Public Health Code). The purchaser (i.e. end user) is required to obtain an import authorization from the France Ministry responsible for Research (as referenced in Article L.1245-5-1 II of the French Public Health Code) or a conservation authorization from the same ministry (as referenced in Article L.1243-4 of the French Public Health Code). By placing an order for this product, you confirm that you have obtained the appropriate import or conservation authorization for the product. For more information regarding the use of human biological samples for research purposes in France, please contact the relevant French authorities.

Información de seguridad

• Clase de almacenamiento: 10 - Combustible liquids
• WGK: WGK 3
• Punto de inflamación (°F): Not applicable
• Punto de inflamación (°C): Not applicable