11363743001
Roche
Nutridoma™-CS
suitable for hybridoma, solution, pkg of 10 mL (50x)
Sinónimos:
serum replacements
Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización
About This Item
UNSPSC Code:
12352207
Productos recomendados
sterility
sterile; 0.2 μm filtered
Quality Level
form
solution
packaging
pkg of 10 mL (50x)
manufacturer/tradename
Roche
concentration
50 ×
technique(s)
cell culture | hybridoma: suitable
impurities
mycoplasma, tested
100 EU/mL endotoxin (LAL test)
solubility
water: miscible
storage temp.
−20°C
Categorías relacionadas
General description
Nutridoma-CS is a serum replacement solution which helps in cell proliferation when added to a suspension. Its major component is β-D xylopyranose which has ring shaped sugar allowing the cells to grow in a suspension even without serum.
Application
Contents
Solution, 50× concentrated, Biochemically defined, filtered through 0.2μm pore size membrane, serum-free medium supplement composed of albumin, insulin, transferrin, several cytokines, a cholesterol source, and other defined organic and inorganic compounds. Nutridoma-CS contains human proteins.
Solution, 50× concentrated, Biochemically defined, filtered through 0.2μm pore size membrane, serum-free medium supplement composed of albumin, insulin, transferrin, several cytokines, a cholesterol source, and other defined organic and inorganic compounds. Nutridoma-CS contains human proteins.
Biochemically defined supplement for the replacement of fetal bovine serum (FBS) in cultures of hybridoma cells. Nutridoma-CS supports the growth of SP2/0-, P3x63Ag8.653-, and NS-1-derived hybridomas. It is specifically formulated to optimize the growth of freshly fused hybridomas during selection and cloning procedures in serum-free cell culture without feeder cells.
Nutridoma™-CS has been used as a supplement in neurobasal medium for culturing human embryonic stem cell (hESC) lines.
Unit Definition
Endotoxin (LAL): 100 EU/ml, mycoplasma tested
Physical form
Solution (50x concentrated, pH 7.4); sterile. The protein concentration is less than 1 mg/ml for 2% (1x) working concentration.
Preparation Note
Working concentration: Nutridoma-CS (50x) is diluted 1:50 (v/v) in basal medium. It is strongly suggested to use RPMI 1640. The final protein concentration is less than 1 mg/ml. The final medium should also contain L-glutamine and β-mercaptoethanol.
Other Notes
For life science research only. Not for use in diagnostic procedures.
Legal Information
Nutridoma is a trademark of Roche
Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
flash_point_f
does not flash
flash_point_c
does not flash
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
¿Ya tiene este producto?
Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
Los clientes también vieron
Debora Steiner et al.
Nature biotechnology, 28(4), 361-364 (2010-03-31)
Undifferentiated human embryonic stem cells (hESCs) are currently propagated on a relatively small scale as monolayer colonies. Culture of hESCs as floating aggregates is widely used for induction of differentiation into embryoid bodies. Here we show that hESC lines can
Mariana Capurro et al.
Gastroenterology, 125(1), 89-97 (2003-07-10)
Early detection of hepatocellular carcinoma (HCC) is critical for successful treatment. However, the differential diagnosis between HCC and benign hepatic lesions is sometimes difficult and new biochemical markers for HCC are required. It has been reported that glypican-3 (GPC3) messenger
Oliver Sterthaus et al.
Cellular reprogramming, 16(3), 185-195 (2014-05-09)
Four normal-karyotype human embryonic stem cell (hESC) lines were generated using the same protocol and maintained under identical conditions. Despite these precautions, gene expression patterns were found to be dissimilar among the four lines. The observed differences were typical of
Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.
Póngase en contacto con el Servicio técnico