11218611910

DNA Molecular Weight Marker VI, DIG-labeled

solution, pkg of 500 μL (10 μg/ml)

• Sinónimos: DNA marker
• UNSPSC Code: 41105335

Propiedades

• form: solution
• Quality Level: 100
• packaging: pkg of 500 μL (10 μg/ml)
• manufacturer/tradename: Roche
• greener alternative product characteristics: Designing Safer Chemicals; Learn more about the Principles of Green Chemistry.
• sustainability: Greener Alternative Product
• greener alternative category: Aligned,
• storage temp.: −20°C

Descripción

General description

DNA Molecular Weight Marker VI, DIG-labeled is a fragment mixture prepared by cleavage of pBR328 DNA and pBR328 DNA with restriction endonucleases Bgl I and Hinf I respectively. The size of the fragment ranges from 0.15 to 2.1 kbp.

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical.  The DIG System was established as a sensitive and cost-effective alternative to using radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization.

Application

DNA Molecular Weight Marker VI, DIG-labeled, has been used as a size standard in Southern blot analysis for nucleic acid labeling and detection. It has also been used as a size standard for amplified DNA.

Components

Ready-to-use solutions in 10mM Tris-HCl, 1mM EDTA, pH 8.0.

Sequence

The mixture contains 12 fragments wit the following base pair lengths: 154/154, 220, 234/234, 298/298, 394, 453, 517, 653, 1033, 1230, 1766, and 2176 bp.
Note: Fragment lengths are derived from computer analysis of the DNA sequence. Depending on the size range of the marker, the smallest fragments will only be visible on overloaded gels.

Other Notes

12 fragments: 154/154; 220; 234/234; 298/298; 394; 453; 517; 653; 1,033; 1,230; 1,766; 2,176 bp
Fragment lengths are derived from computer analysis of the DNA sequence. Depending on the size range of the marker, the smallest fragments will only be visible on overloaded gels.

For life science research only. Not for use in diagnostic procedures.

Información de seguridad

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: nwg
• flash_point_f: No data available
• flash_point_c: No data available