10109045001
Roche
Pyruvate Kinase (PK)
from rabbit muscle
Sinónimos:
PK
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origen biológico
rabbit muscle
Nivel de calidad
Formulario
solution
actividad específica
~200 units/mg protein (At 25 °C with PEP as the substrate.)
envase
pkg of 1 mL (10 mg)
fabricante / nombre comercial
Roche
pH óptimo
7.0-7.5
Condiciones de envío
dry ice
temp. de almacenamiento
−20°C (−15°C to −25°C)
Categorías relacionadas
Descripción general
Pyruvate kinase has a molar mass of 237,000 and exists as a tetramer. Each polypeptide chain of this tetramer has a molar mass of 57,200. The enzyme contains two identical catalytic particles called protomers. Each of these protomers contains two polypeptide chains. Each protomer contains one site each for Mn2+ and phosphoenolpyruvate.[1]
Especificidad
Specific activity: Approximately 200 U/mg at +25°C with PEP as the substrate.
Aplicación
Pyruvate Kinase (PK) has been used in the kinase assay.[4]
Acciones bioquímicas o fisiológicas
Pyruvate kinase catalyzes the irreversible conversion of P-enolpyruvate and ADP to pyruvate and ATP with the utilization of a proton. The first step is the transfer of phosphate group from P-enolpyruvate to ADP with the formation of bound enolate of pyruvate and ATP. In the second step, a proton is added to enolate to generate the keto form of pyruvate. The enzyme also exhibits other activities, such as ATP- and bicarbonate-dependent ATPase, phosphorylation of fluoride and hydroxylamine, ATP-dependent phosphorylation of glycolate, and decarboxylation of oxaloacetate.[5]
Calidad
Contaminants: <0.001% GK, <0.002% HK, “NADH oxidase”, and ATPase, each, <0.01% enolase, LDH, and myokinase, each.
Forma física
Solution in 50% glycerol (v/v), pH approximately 6
Nota de preparación
Activator: PK requires Mg2+ (or Mn2+, Co2+) and K+ (or NH4+, Rb+) for full activity.
Otras notas
For life science research only. Not for use in diagnostic procedures.
Código de clase de almacenamiento
12 - Non Combustible Liquids
Clase de riesgo para el agua (WGK)
WGK 1
Punto de inflamabilidad (°F)
No data available
Punto de inflamabilidad (°C)
No data available
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