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Merck
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S1-M

Sigma-Aldrich

Human Serum, Normal

Sinónimos:

Normal Human Serum

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About This Item

UNSPSC Code:
41105901
eCl@ss:
26020100
NACRES:
NA.71
biological source:
human
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biological source

human

antibody form

serum

manufacturer/tradename

Chemicon®

technique(s)

blocking: suitable (interfering antibodies)

General description

Normal human serum collected off the clot from healthy normal humans. Each donor unit was tested and found negative for HIV 1&2, HIV-Ag, HCV, HBsAg, and RPR by FDA approved methods.

Application

Normal Human Serum has been used as a reference or control in degradation assays involving DNase I and MNase treatments, providing a comparison for assessing the impact of these enzymes on the DHM structures.[1]

Physical form

Nonsterile liquid. Contains no preservatives.

Filtered through a 0.2μ absolute membrane filter before bottling.

Storage and Stability

Store frozen at -20°C for up to 12 months. Avoid repeated freeze/thaw cycles.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Midori L Wasielewski et al.
International journal of molecular sciences, 24(4) (2023-02-26)
This study analyzed the nuclease- and serum-driven degradation of millimeter-scale, circular DNA-histone mesostructures (DHMs). DHMs are bioengineered chromatin meshes of defined DNA and histone compositions designed as minimal mimetics of physiological extracellular chromatin structures, such as neutrophil extracellular traps (NETs).

Questions

1–6 of 6 Questions  

  1. Is dialyzed human serum available?

    1 answer

    1. Dialyzed serum is not sold as a product. However, normal human serum (cat #s S1-LITER or S1-100ML) is available.
      Additionally, we provide various products that allow the client to dialyze the serum. Depending on the volume of serum to be dialyzed, any of the following products will work:
      •D-Tubes or Amicon Ultra for small volumes
      •Centricon Plus-70 for centrifuging 70 mL samples (cat # UFC701008)
      •Stirred cells along with 10 kDa MWCO ultrafiltration membrane discs for volumes up to 400 mL

      Helpful?

  2. Is the product "Human Serum, Normal (S1-100ML)" Male only, Female only or Male/Female mix?

    1 answer

    1. This product is pooled human serum, typically consisting of hundreds of male and female donors.

      Helpful?

  3. Is there any detectable thrombin in this serum?

    1 answer

    1. Unfortunately, this product is not tested for thrombin.

      Helpful?

  4. Hi, wonder if this is a pooled serum from multiple donors or a single bottle is from a single donor? thanks.

    1 answer

    1. This serum product is pooled from multiple donors. The typical batch varies but can range from 400 to 500 donors.

      Helpful?

  5. I would like to know whether it has been inactivated as I intend to use the complement components in the serum to activate the complement system in vitro. Additionally, does this serum have been previously used in complement activation studies？

    1 answer

    1. This product has not been inactivated, however it is not tested for use in compliment studies. The product may or may not be suitable for this application. See the link below for a product that may be of interest:
      https://www.sigmaaldrich.com/product/sigma/c9473.

      Helpful?

  6. Hi! I was wondering how this serum is collected and what storage agents/additives were used in the collection tubes/bags. E.g. silica in Vacutainer SST tubes

    1 answer

    1. As it states in the "Description" section, normal human serum is collected off the clot from healthy normal humans. It is a nonsterile liquid with no preservatives.

      Helpful?

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