NE1013
Anti-PGP9.5 (176-191) Rabbit pAb
liquid, Calbiochem®
Sinónimos:
Anti-Neuron Cytoplasmic Protein 9.5, Anti-Ubiquitin Thiolesterase L1, Anti-UCHL1
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About This Item
Código UNSPSC:
12352203
NACRES:
NA.41
En este momento no podemos mostrarle ni los precios ni la disponibilidad
Productos recomendados
origen biológico
rabbit
Nivel de calidad
forma del anticuerpo
serum
tipo de anticuerpo
primary antibodies
clon
polyclonal
Formulario
liquid
contiene
≤0.1% sodium azide as preservative
reactividad de especies
porcine, human, rat, mouse
fabricante / nombre comercial
Calbiochem®
condiciones de almacenamiento
OK to freeze
avoid repeated freeze/thaw cycles
isotipo
IgG
Condiciones de envío
wet ice
temp. de almacenamiento
−20°C
modificación del objetivo postraduccional
unmodified
Información sobre el gen
human ... UCHL1(7345)
Descripción general
Rabbit polyclonal antibody supplied as undiluted serum. Recognizes the ~26 kDa PGP9.5 protein.
Recognizes the ~26 kDa PGP9.5 protein in mouse dorsal root nerve fibers.
This Anti-PGP9.5 (176-191) Rabbit pAb is validated for use in Immunoblotting, Frozen Sections for the detection of PGP9.5 (176-191).
Inmunógeno
a synthetic peptide (ASSEDTLLKDAAKVCR) corresponding to amino acids 176-191 of PGP9
Aplicación
Immunoblotting (1:1000)
Frozen Sections (1:2000, fluorescence)
Advertencia
Toxicity: Standard Handling (A)
Forma física
Undiluted serum.
Reconstitución
Following initial thaw, aliquot and freeze (-20°C).
Nota de análisis
Positive Control
Mouse dorsal root nerve fibers
Mouse dorsal root nerve fibers
Otras notas
Caballero, O.L., et al. 2002. Oncogene21, 3003.
Tezel, E., et al. 2000. Clin. Cancer Res.6, 4764.
Tezel, E., et al. 2000. Clin. Cancer Res.6, 4764.
PGP9.5 is also overexpressed in some cancers.
Immunofluorescence protocol
Care should be taken so that the incubation solutions do not evaporate. It is recommended that incubations be performed in a humidified chamber to prevent evaporation. Washing steps should be done in large volumes of phosphate buffered saline (PBS).
1. Warm slides to room temperature.
2. Re-hydrate with PBS for 10-15 min.
3. Remove PBS and incubate in blocking buffer (PBS containing 3% normal donkey serum, 1% bovine serum albumin, 0.3% Triton™ X-100 detergent, and 0.01% NaN3, pH 7.45) for 1 h at room temperature.
4. Dilute primary antibody in blocking buffer. Store diluted antibody at 4°C until use.
5. Remove blocking buffer and add diluted primary antibody.
6. Incubate with primary antibody at 4°C for 18-48 h.
7. Remove primary antibody and wash three times for 10 min with PBS.
8. Dilute secondary antibody in blocking buffer to appropriate dilution (follow manufacture′s recommendation). Store at 4°C until use.
9. Incubate slides in diluted secondary antibody solution for one hour at room temperature.
10. Remove secondary antibody and wash three times for 10 min with PBS.
11. Coverslip slides with appropriate mounting media.
Immunofluorescence protocol
Care should be taken so that the incubation solutions do not evaporate. It is recommended that incubations be performed in a humidified chamber to prevent evaporation. Washing steps should be done in large volumes of phosphate buffered saline (PBS).
1. Warm slides to room temperature.
2. Re-hydrate with PBS for 10-15 min.
3. Remove PBS and incubate in blocking buffer (PBS containing 3% normal donkey serum, 1% bovine serum albumin, 0.3% Triton™ X-100 detergent, and 0.01% NaN3, pH 7.45) for 1 h at room temperature.
4. Dilute primary antibody in blocking buffer. Store diluted antibody at 4°C until use.
5. Remove blocking buffer and add diluted primary antibody.
6. Incubate with primary antibody at 4°C for 18-48 h.
7. Remove primary antibody and wash three times for 10 min with PBS.
8. Dilute secondary antibody in blocking buffer to appropriate dilution (follow manufacture′s recommendation). Store at 4°C until use.
9. Incubate slides in diluted secondary antibody solution for one hour at room temperature.
10. Remove secondary antibody and wash three times for 10 min with PBS.
11. Coverslip slides with appropriate mounting media.
Información legal
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow
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Código de clase de almacenamiento
10 - Combustible liquids
Clase de riesgo para el agua (WGK)
WGK 1
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