MABE369

Anti-Suv4–20h2 Antibody, clone 6B9

clone 6B9, from rat

• Sinónimos: Histone-lysine N-methyltransferase SUV420H2, Lysine N-methyltransferase 5C, Suppressor of variegation 4-20 homolog 2, Su(var)4-20 homolog 2, Suv4-20h2
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• clone: 6B9, monoclonal
• application: WB
• species reactivity: human
• technique(s): western blot: suitable
• citations: 2

Propiedades

• biological source: rat
• Quality Level: 100
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 6B9, monoclonal
• species reactivity: human
• technique(s): western blot: suitable
• isotype: IgG2bκ
• NCBI accession no.: NP_116090
• UniProt accession no.: Q86Y97
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... SUV420H2(84787)

Descripción

General description

Suppressor of variegation 4-20 homolog 2 (Suv4-20h2), also known as Lysine N-methyltransferase 5C (KMT5C), is a Histone-lysine N-methyltransferase that specifically trimethylates Lys-20 of histone H4. Suv4-20h2 mainly functions in pericentric heterochromatin regions, thereby playing a central role in the establishment of constitutive heterochromatin in these regions. In mammals, Histone H4 Lys-20 trimethylation (H4K20me3) is one of the most common chromatin modifications and it has been implicated in various biological processes, including heterochromatin formation, telomere function, imprinting, X inactivation, DNA damage repair and cancer progression.

Immunogen

GST-tagged recombinant protein corresponding to human Suv4-20h2.

Application

Detect the Suv4–20h2 protein using this Anti-Suv4–20h2, clone 6B9 validated for use in WB.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Chromatin Biology

Quality

Evaluated by Western Blot in H9 human embryonic stem cell lysate.

Western Blot Analysis: 4.0 µg/mL of this antibody detected Suv4-20h2 in 10 µg of H9 human embryonic stem cell lysate.

Target description

~53 kDa observed. Uniprot describes 2 isoforms produced by alternative splicing at ~11 kDa and ~15 kDa

Physical form

Format: Purified

Protein G Purified

Purified rat monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
H9 human embryonic stem cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable