MAB1202
Anti-CD42a Antibody, platelet lb-IX, clone FMC-25
clone FMC-25, Chemicon®, from mouse
Sinónimos:
Anti-CD42a, Anti-GPIX
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Productos recomendados
biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
FMC-25, monoclonal
species reactivity
human
manufacturer/tradename
Chemicon®
technique(s)
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
isotype
IgG1
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... GP9(2815)
Categorías relacionadas
Specificity
This antibody belongs to CD42a (assigned at the Fourth International Workshop on Leukocyte Differentiation Antigens, Vienna, 1989) (Knapp, 1989), and binds to the 22,000 dalton IX subunit of the glycoprotein Ib-IX complex (Zola et al., 1984; Berndt et al., 1985a,b). FMC-25 failed to bind to platelets from patients with Bernard-Soulier Syndrome (Berndt et al., 1983). The antibody is specific for cells of the platelet megakaryocyte lineage. It reacts with a proportion of acute myeloid leukemias being useful in identifying those of the megakaryoblastic subtype (San Miguel et al., 1985, 1986).
Cell reactivity:
The antibody is specific for cells of the platelet megakaryocyte lineage. It reacts with a proportion of acute myeloid leukemias being useful in identifying those of the megakaryoblastic subtype(San Miguel et al., 1985, 1986).
Cell reactivity:
The antibody is specific for cells of the platelet megakaryocyte lineage. It reacts with a proportion of acute myeloid leukemias being useful in identifying those of the megakaryoblastic subtype(San Miguel et al., 1985, 1986).
Immunogen
Epitope: platelet lbalpha complex
Peripheral blood mononuclear cells isolated on Ficoll® hypaque.
Application
Anti-CD42a Antibody, platelet lb-IX, clone FMC-25 detects level of CD42a & has been published & validated for use in FC, IC, IH.
Research Category
Inflammation & Immunology
Inflammation & Immunology
Research Sub Category
Immunoglobulins & Immunology
Immunoglobulins & Immunology
The glycoprotein Ib-IX complex on the surface of normal platelets is a heterotrimer of Ib-alpha, Ib-beta and IX subunits (Berndt, 1989). Ib-IX complex is an essential component of platelet adhesion to vascular subendothelium and is also important in the maintenance of platelet shape (Berndt et al., 1988).
This antibody is suitable for enumeration of platelets by flow cytometry and immunoperoxidase staining of frozen tissue sections or air dried smear preparations (San Miguel et al., 1986), and for identification of megakaryoblasts in acute myeloid leukemia or acute myelofibrosis (San Miguel et al., 1985; San Miguel et al., 1986).
SUGGESTED USAGE DILUTION
1. Flow cytometry and indirect immunofluorescence 1:25
Dilute with isotonic buffer. Use 50 μl of diluted antibody per 1 x 10E6 platelets in 100 μl of buffer.
2. Indirect immunoperoxidase staining - the final dilution will depend on the assay conditions and detection system employed. However, a dilution of at least 1:20 will be applicable to most commonly used systems.
This antibody is suitable for enumeration of platelets by flow cytometry and immunoperoxidase staining of frozen tissue sections or air dried smear preparations (San Miguel et al., 1986), and for identification of megakaryoblasts in acute myeloid leukemia or acute myelofibrosis (San Miguel et al., 1985; San Miguel et al., 1986).
SUGGESTED USAGE DILUTION
1. Flow cytometry and indirect immunofluorescence 1:25
Dilute with isotonic buffer. Use 50 μl of diluted antibody per 1 x 10E6 platelets in 100 μl of buffer.
2. Indirect immunoperoxidase staining - the final dilution will depend on the assay conditions and detection system employed. However, a dilution of at least 1:20 will be applicable to most commonly used systems.
Physical form
Format: Purified
Purified immunoglobulin. The antibody is supplied in 25mM Tris-HCl, 0.4M NaCl, pH 8.0 containing 0.2% bovine serum albumin and 0.1% sodium azide. The characteristics of each lot are tested by electrophoresis and flow cytometry.
Storage and Stability
Store at 2 to 8°C, for up to 6 months. For prolonged periods, store below -20°C in undiluted aliquots. AVOID REPEATED FREEZE/THAW CYCLES.
WARNING: The monoclonal reagent solution contains 0.1% sodium azide as a preservative. Due to potential hazards arising from the build up of this material in pipes, spent reagent should be disposed of with liberal volumes of water.
WARNING: The monoclonal reagent solution contains 0.1% sodium azide as a preservative. Due to potential hazards arising from the build up of this material in pipes, spent reagent should be disposed of with liberal volumes of water.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Ficoll is a registered trademark of Cytiva
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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signalword
Warning
hcodes
pcodes
Hazard Classifications
Met. Corr. 1
Storage Class
8B - Non-combustible corrosive hazardous materials
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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Rong Yan et al.
Cell death & disease, 12(11), 955-955 (2021-10-18)
Platelets are generated from the cytoplasm of megakaryocytes (MKs) via actin cytoskeleton reorganization. Zyxin is a focal adhesion protein and wildly expressed in eukaryotes to regulate actin remodeling. Zyxin is upregulated during megakaryocytic differentiation; however, the role of zyxin in
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