HCCBP2MAG-58K

MILLIPLEX^® Human Circulating Cancer Biomarker Magnetic Bead Panel 2 - Cancer Multiplex Assay

Circulating Cancer Biomarker Bead-Based Multiplex Assays using the Luminex technology enables the simultaneous analysis of multiple oncology biomarkers in various tumor types in human serum, plasma and cell culture samples.

• UNSPSC Code: 12161503
• eCl@ss: 32161000
• NACRES: NA.84

Propiedades

• Quality Level: 200
• species reactivity: human
• manufacturer/tradename: Milliplex^®
• assay range: accuracy: 92-107%; standard curve range: 0.01-50 ng/mL; (Extracellular matrix protein 1); standard curve range: 0.48-2,000 ng/mL; (All other biomarkers)
• technique(s): multiplexing: suitable
• detection method: fluorometric (Luminex xMAP)
• shipped in: wet ice

Descripción

General description

Circulating cancer biomarkers give insight beyond intracellular oncogenesis and into how the body responds to the presence of a tumor, the dysregulation of homeostasis, and the relationship between a tumor and its environment. Multianalyte panels have become essential tools that enable researchers to measure cancer biomarkers simultaneously, teasing out these relationships and applying them to understand tumor biology.

MILLIPLEX^® Circulating Cancer Biomarker Panel 2 is a 4-plex kit that can be used for the simultaneous quantification of any or all of the following analytes in serum, plasma, tissue/cell lysate and culture supernatant samples: Extracellular matrix Protein 1 (ECM1), Antithrombin III, Vitamin D binding protein, and Complement Factor H (CFH). This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate.

The Luminex^® xMAP^® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.

Panel Type: Circulating Cancer

Application

• Analytes: Antithrombin III, Complement Factor H (CFH), Extracellular matrix Protein 1 (ECM1), Vitamin D binding protein
• Recommended Sample Type: Human serum, plasma or tissue/cell culture supernatants or lysates
• Note: Serum has a thrombin/antithrombin III complex bound to vitronectin. To accurately quantify antithrombin III concentrations we recommend using plasma samples because they lack the vitronectin/thrombin/antithrombin complex.
• Recommended Sample Dilution: 25 μL of a 1:10,000 dilution of plasma or serum, or 25 μL of tissue/cell culture sample diluted in an appropriate control medium (dilution factors may vary)
• Assay Run Time: Overnight (16-18 hours) at 2-8°C
• Research Category: Cancer

Features and Benefits

Design your multiplex kit by choosing available analytes within this panel.

Components

Human Circulating Cancer Biomarker Panel 2 Standard (HCC-8058-2)

Human Circulating Cancer Biomarker Panel 2 Quality Controls 1 and 2 (HCC-6058-2)

Set of one 96-Well Plate with 2 Sealers

Bead Diluent (LBD-4)

Assay Buffer (L-AB)
Note: Contains 0.08% Sodium Azide

10X Wash Buffer (L-WB)
Note: Contains 0.05% Proclin

Human Circulating Cancer Biomarker Panel 2 Detection Antibodies (HCC-1058)

Streptavidin-Phycoerythrin (L-SAPE 6)

Other Notes

Sensitivity: Refer to kit protocol for sensitivities of individual biomarkers.

Legal Information

Luminex is a registered trademark of Luminex Corp

MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

xMAP is a registered trademark of Luminex Corp

Información de seguridad

• pictograms: GHS06,GHS08,GHS05,GHS09
• signalword: Danger
• hcodes: H302 + H332,H311,H317,H318,H373,H411
• pcodes: P273 - P280 - P302 + P352 + P312 - P304 + P340 + P312 - P305 + P351 + P338 - P314
• Hazard Classifications: Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Skin Sens. 1 - STOT RE 2
• target_organs: Respiratory Tract
• Storage Class: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects