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ABN352

Sigma-Aldrich

Anti-NENF Antibody

from rabbit, purified by affinity chromatography

Sinónimos:

NENF, Neuron derived neurotrophic factor, neudesin, CIR2, SPUF, SCIRP10

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

rat, human

technique(s)

immunohistochemistry: suitable
western blot: suitable

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... NENF(29937)

General description

NENF is a secreted protein that is expressed in neurons but not glial cells of the brain. This protein has neurotrophic activity in primary cultured mouse neurons but not mitogenic activity in primary cultured mouse astrocytes. NENF activated mitogen-activated protein (MAP) and phosphotidylinositol-3 kinase pathways and could be inhibited by pertussis toxin but not receptor tyrosine kinases, suggesting that NENF acts through a Gi/Go-protein-coupled receptor.

Immunogen

KLH-conjugated linear peptide corresponding to human NENF.

Application

Detect the NENF protein using this Anti-NENF validated for use in WB & IHC.
Immunohistochemistry Analysis: 10 µg/mL of this antibody from a representative lot detected NENF in rat kidney tissue.
Research Category
Neuroscience
Research Sub Category
Neuronal & Glial Markers

Quality

Evaluated by Western Blot in human kidney tissue lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected NENF in 15 µg of human kidney tissue lysate.

Target description

~37 kDa observed

Physical form

Antigen Affinity Purified
Purified rabbit polyclonal in buffer containing PBS and 0.02% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Human kidney tissue lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificados de análisis (COA)

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