ABE797

Anti-APC8 Antibody

from rabbit, purified by affinity chromatography

• Sinónimos: Cell division cycle protein 23 homolog, Anaphase-promoting complex subunit 8, APC8, Cyclosome subunit 8
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: rabbit
• Nivel de calidad: 100
• forma del anticuerpo: affinity isolated antibody
• tipo de anticuerpo: primary antibodies
• clon: polyclonal
• purificado por: affinity chromatography
• reactividad de especies: human
• técnicas: immunocytochemistry: suitable; western blot: suitable
• Nº de acceso UniProt: Q9UJX2
• Condiciones de envío: wet ice
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... CDC23(8697)

Descripción

Descripción general

Cell division cycle protein 23 homolog, also known as Anaphase-promoting complex subunit 8 (APC8) or Cyclosome subunit 8, and encoded by the gene name CDC23 or ANAPC8, is a highly conserved component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. APC/C is responsible for degrading anaphase inhibitors, mitotic cyclins, and spindle-associated proteins ensuring that events of mitosis take place in proper sequence. The individual APC/C components mRNA and protein levels are expressed at approximately the same levels in most tissues and cell lines, suggesting that they perform their functions as part of a complex. In Drosophila, silencing of APC8 results in developmental delay and pupal lethality with elevated levels of apoptosis, high mitotic index, and delayed or blocked mitosis.

Inmunógeno

Epitope: center region

KLH-conjugated synthetic peptide corresponding to the center region of human APC8.

Aplicación

Detect APC8 using this Anti-APC8 Antibody validated for use in western blotting & ICC.

Immunocytochemistry Analysis: 5 μg/mL from a representative lot detected APC8 in K562 cells.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Cell Cycle, DNA Replication & Repair

Calidad

Evaluated by Western Blotting in K562 cell lysate.

Western Blotting Analysis: 2 μg/mL of this antibody detected APC8 in K562 cell lysate.

Descripción de destino

~55 kDa observed. Uncharacterized bands may be observed in some lysate(s). Uniprot describes 3 isoforms produced by alternative splicing at ~17 kDa, ~56 kDa, and ~69 kDa

Forma física

Antigen Affinity Purified

Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.

Almacenamiento y estabilidad

Stable for 1 year at 2-8°C from date of receipt.

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 2
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable