ABC242

Anti-CRKL Antibody

from rabbit, purified by affinity chromatography

• Sinónimos: Crk-like protein
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• origen biológico: rabbit
• Nivel de calidad: 100
• forma del anticuerpo: affinity isolated antibody
• tipo de anticuerpo: primary antibodies
• clon: polyclonal
• purificado por: affinity chromatography
• reactividad de especies: rat, human, mouse
• reactividad de especies (predicha por homología): rhesus macaque (based on 100% sequence homology)
• técnicas: immunoprecipitation (IP): suitable; western blot: suitable
• Nº de acceso NCBI: NP_005198
• Nº de acceso UniProt: P46109
• Condiciones de envío: wet ice
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... CRKL(1399)

Descripción

Descripción general

Crk-like protein (CRKL) is a 39 kDa adaptor protein with one SH2 and two SH3 binding domains. CRKL is the major protein which is tyrosine phosphorylated in response to BCR/ABL (chronic myelogenous leukemia) and growth factor activation. In myeloid cells, it binds to proteins such as paxillin, p130cas and p120 cbl. CRKL aslo appears to bind ABL and SOS.

Inmunógeno

KLH-conjugated linear peptide corresponding to human CRKL.

Aplicación

Detect Crk-like protein using this rabbit polyclonal antibody, Anti-CRKL Antibody validated for use in western blotting & IP.

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

Western Blotting Analysis: 0.25 µg/mL from a representative lot detected CRKL in 10 µg of NIH3T3 and L6 cell lysate.
Immunoprecipitation Analysis: 10 µg from a representative lot immunoprecipitated CRKL in 500 µg of Jurkat cell lysate.

Calidad

Evaluated by Western Blotting in Jurkat cell lysate.

Western Blotting Analysis: 0.25 µg/mL of this antibody detected CRKL in 10 µg of Jurkat cell lysate.

Descripción de destino

~39 kDa observed. Uncharacterized band(s) may appear in some lysates.

Forma física

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Almacenamiento y estabilidad

Stable for 1 year at 2-8°C from date of receipt.

Otras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable