AB5666P

Anti-Neurite Outgrowth Inhibitor A/B/C Antibody

Chemicon^®, from rabbit

• Sinónimos: Nogo-A/B/C
• UNSPSC Code: 12352203
• eCl@ss: 32160702

Propiedades

• biological source: rabbit
• Quality Level: 100
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: human, mouse, rat
• manufacturer/tradename: Chemicon^®
• technique(s): ELISA: suitable; western blot: suitable
• NCBI accession no.: NM_007008.2; NM_020532.4; NM_153828.2; NM_207520.1; NM_207521.1
• UniProt accession no.: Q9NQC3
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... RTN4(57142)

Descripción

Specificity

Recognizes human Neurite Outgrowth Inhibitor Protein A/B/C (Nogo-A/B/C). The immunogen peptide is within the 25 amino acid region identified for the inhibition of neurite outgrowth (Chen et al. 2000; GrandPre et al. 2000; Goldberg & Barres 2000; Prinijha et al. 2000; Tessier-Lavigne & Goodman 2000; Nagase et al. 1998).The immunogen shows no significant sequence homology with other known proteins.

SPECIES REACTIVITIES: The immunogen peptide is 92% conserved in rat Nogo or rat foocen-m1.2 protein (Chen et al. 2000; GrandPre et al. 2000; Goldberg & Barres 2000; Prinijha et al. 2000; Tessier-Lavigne & Goodman 2000; Nagase et al. 1998). Reactivity with other species has not been confirmed.

Immunogen

A 13 amino acid peptide between the putative 2nd-3rd transmembrane domain of human Nogo-A (Chen et al. 2000; GrandPre et al. 2000; Goldberg & Barres 2000; Prinijha et al. 2000; Tessier-Lavigne & Goodman 2000; Nagase et al. 1998).

Application

Anti-Neurite Outgrowth Inhibitor A/B/C Antibody is an antibody against Neurite Outgrowth Inhibitor A/B/C for use in ELISA & WB.

Research Category
Neuroscience

Research Sub Category
Neurochemistry & Neurotrophins

Growth Cones & Axon Guidance

Western blot: 1-10 μg/mL using ECL. The calculated molecular weight of Nogo-A is approximately 135 kDa. However a molecular weight of approximately 180 kDa has been reported for full length Nogo-A (Chen et al. 2000; GrandPre et al. 2000; Goldberg & Barres 2000; Prinijha et al. 2000; Tessier-Lavigne & Goodman 2000; Nagase et al. 1998).



ELISA: 1:10,000-1:100,000 using 50-100 ng control peptide per well.

Optimal working dilutions must be determined by the end user.

Physical form

Liquid in PBS containing 0.1% BSA and 0.05% sodium azide.

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Storage Class: 10 - Combustible liquids