345872

Glial Derived Neurotrophic Factor, Human, Recombinant, E. coli

• Sinónimos: rhGDNF
• Código UNSPSC: 12352202

Propiedades

• Ensayo: ≥97% (SDS-PAGE)
• Nivel de calidad: 100
• Formulario: lyophilized
• fabricante / nombre comercial: Calbiochem^®
• condiciones de almacenamiento: OK to freeze; desiccated
• solubilidad: aqueous buffer: soluble (most); water: soluble
• Condiciones de envío: ambient
• temp. de almacenamiento: −20°C

Descripción

Descripción general

Recombinant, human glial derived neutotrophic factor expressed in E. coli. A heterogeneously glycosylated growth promoting factor that enhances neuronal survival of dopaminergic midbrain neurons. Activates receptor tyrosine kinase Ret and promotes kidney morphogenesis. Also an activator of MAP kinase. Its biological activity is destroyed by reduction of disulfide bonds.

Recombinant, human glial derived neutotrophic factor expressed in E. coli. Growth promoting factor that enhances neuronal survival of dopaminergic midbrain neurons. A heterogeneously glycosylated basic protein. Its biological activity is destroyed by the reduction of disulfide bonds.

Acciones bioquímicas o fisiológicas

fully biologically active when compared to native GDNF by dose-dependent ³H-dopamine uptake assay

Advertencia

Toxicity: Standard Handling (A)

Nota de preparación

The recommended stock solution is 1 µg/100µl. For more dilute samples, add a stabilizing protein such as 0.1% BSA.

Reconstitución

Following reconstitution, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 3 months at -20°C.

Otras notas

Vega, Q.C., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 10657.
Worby, C.A., et al. 1996. J. Biol. Chem. 271, 23619.
Lin, L.F., et al. 1994. J. Neurochem. 63, 758.
Lev-Fen, H., et al. 1993. Science260, 1130.

Información legal

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany