204860

Complement C3b, Human

Complement C3b, Human, is a native C3b complement component. It is a glycoprotein composed of the modified C3-α chain (αʹ) (M.W. 105,000) and the intact C3-β chain (M.W. 75,000).

• Sinónimos: C3b, Complement C3
• Código UNSPSC: 12352202
• NACRES: NA.25

Propiedades

• Nivel de calidad: 100
• Ensayo: ≥90% (SDS-PAGE)
• Formulario: liquid
• fabricante / nombre comercial: Calbiochem^®
• condiciones de almacenamiento: OK to freeze; avoid repeated freeze/thaw cycles
• impurezas: IgG, IgA, IgM, C5, Factor H, or Factor I, trace
• Condiciones de envío: wet ice
• temp. de almacenamiento: −70°C

Descripción

Descripción general

Native, human C3b complement component. Cleavage of the C3-α chain at peptide bond 77 by either of the complement C3 convertase enzymes results in production of C3a (M.W. 9083) and C3b (M.W. 180,000) fragments. The C3b fragment is a glycoprotein composed of the modified C3-α chain (α′) (M.W. 105,000) and the intact C3-β chain (M.W. 75,000). Nascent C3b has the transient ability to form a covalent ester bond with a variety of target surfaces. Once bound to target surfaces C3b becomes an essential subunit of both the classical and alternative pathway C5 cleaving enzymes. In addition, surface-bound C3b has opsonic and immune adherence activities which are mediated via binding to CR1 (CD35) complement receptors.

Advertencia

Toxicity: Standard Handling (A)

Forma física

In PBS, pH 7.2, filtered through a 0.22 µm filter.

Nota de preparación

Prepared from serum that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.

Reconstitución

Following initial thaw, aliquot and freeze (-70°C).

Otras notas

Wong, W.W. and Fearon, D.T. 1987. Methods Enzymol.150, 579.
Arnout, M.A., et al. 1981. J. Immunol.127, 1348.

Información legal

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable