05-1352
Anti-Histone H2B Antibody, clone 5HH2-2A8
ascites fluid, clone 5HH2-2A8, from mouse
Sinónimos:
H2B, Histone H2B, histone 2, H2be, histone cluster 2, H2be, H2B histone family, member Q
Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Productos recomendados
biological source
mouse
Quality Level
antibody form
ascites fluid
antibody product type
primary antibodies
clone
5HH2-2A8, monoclonal
species reactivity
mouse, human, rat
technique(s)
immunocytochemistry: suitable
western blot: suitable
isotype
IgG1κ
GenBank accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... H2BC21(8349)
mouse ... H2Bc21(319190)
General description
Histones are basic nuclear proteins that are responsible for the nucleosome structure of chromatin in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which DNA is wrapped in repeating units, called nucleosomes. Histone H2B is one of the main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H2B is involved with the structure of the nucleosomes of the ′beads on a string′ structure.
Specificity
Antibody recognizes Histone H2B near the C-terminus.
Immunogen
Epitope: C-terminus
Ovalbumin-conjugated synthetic linear peptide corresponding to the C-terminus of Histone H2B.
Application
Anti-Histone H2B Antibody, clone 5HH2-2A8 is a Mouse Monoclonal Antibody for detection of Histone H2B also known as Histone H2B, histone 2 H2be & has been validated in WB, ICC.
Immunocytochemistry:
A 1:500 dilution of a representative lot of this antibody detected Histone H2B in A431, HeLa and NIH/3T3 cells.
A 1:500 dilution of a representative lot of this antibody detected Histone H2B in A431, HeLa and NIH/3T3 cells.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Histones
Histones
Quality
Evaluated by western blot in HepG2 cell lysate.
Western Blot Analysis:
A 1:1,000-1:2,000 dilution of this antibody detected Histone H2B in 10 µg of HepG2 cell lysate.
Western Blot Analysis:
A 1:1,000-1:2,000 dilution of this antibody detected Histone H2B in 10 µg of HepG2 cell lysate.
Target description
~15 kDa
Linkage
Replaces: MABE15
Physical form
Mouse monoclonal IgG1κ ascites with 0.05% sodium azide.
Unpurified
Storage and Stability
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Control
HepG2 cell lysate, L6 cell lysate, Mouse Brain Lysate
HepG2 cell lysate, L6 cell lysate, Mouse Brain Lysate
Legal Information
GenBank is a registered trademark of United States Department of Health and Human Services
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Not finding the right product?
Try our Herramienta de selección de productos.
Storage Class
12 - Non Combustible Liquids
wgk_germany
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
¿Ya tiene este producto?
Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
Usha P Andley et al.
BMC research notes, 13(1), 315-315 (2020-07-04)
Understanding the mechanisms of cataract formation is important for age-related and hereditary cataracts caused by mutations in lens protein genes. Lens proteins of the crystallin gene families α-, β-, and γ-crystallin are the most abundant proteins in the lens. Single
USP7 cooperates with SCML2 to regulate the activity of PRC1.
Lecona, E; Narendra, V; Reinberg, D
Molecular and cellular biology null
Louis Legoff et al.
Environment international, 152, 106472-106472 (2021-03-13)
Chlordecone (CD), also known as Kepone, is an organochlorine insecticide that has been used in banana crops in the French West Indies. Due to long-term contamination of soils and water, the population is still exposed to CD. Exposure to CD
Valery F Thompson et al.
Biochemistry, 57(51), 7021-7032 (2018-11-30)
Purified recombinant FUsed in Sarcoma (FUS) assembles into an oligomeric state in an RNA-dependent manner to form large condensates. FUS condensates bind and concentrate the C-terminal domain of RNA polymerase II (RNA Pol II). We asked whether a granule in
Yang Lim et al.
Science (New York, N.Y.), 381(6664), eadi3448-eadi3448 (2023-08-17)
CDC45-MCM2-7-GINS (CMG) helicase assembly is the central event in eukaryotic replication initiation. In yeast, a multi-subunit "pre-loading complex" (pre-LC) accompanies GINS to chromatin-bound MCM2-7, leading to CMG formation. Here, we report that DONSON, a metazoan protein mutated in microcephalic primordial
Artículos
Loading controls in western blotting application.
Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.
Póngase en contacto con el Servicio técnico