A sensitive assay for beta-ureidopropionase based on derivatization of the reaction product beta-alanine with phenylisothiocyanate has been developed. Purification of the resulting phenylthiocarbamoyl-beta-alanine is achieved on a LiChrospher 100 C18 reversed-phase high-performance liquid chromatography column using an isocratic elution system.
Simple method for the quantitative analysis of dihydropyrimidines and N-carbamyl-beta-amino acids in urine.
A H van Gennip et al.
Advances in experimental medicine and biology, 309B, 15-19 (1991-01-01)
Improved colorimetric procedure for quantitating N-carbamoyl-beta-alanine with minimum dihydrouracil interference.
Journal of inherited metabolic disease, 28(6), 1109-1122 (2006-01-26)
To evaluate the significance of inborn metabolic disorders of the pyrimidine degradation pathway, 450 children with unspecific neurological symptoms were comprehensively studied; 200 healthy children were recruited as controls. Uracil and thymine as well as their degradation products in urine
Two modified procedures for the colorimetric quantitation of citrulline aimed primarily at the assay of ornithine transcarbamylase were developed. Both methods give highly reproducible results in a short period of time since color is developed in 25 min at 100
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