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G005611

HPLC Analysis of Withania Standards on Discovery® C18

application for HPLC

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About This Item

technique(s)

HPLC: suitable

test parameters

column: Discovery C18, 25 cm x 4.6 mm I.D., 5 μm particles (504971)
mobile phase: [A] 1 mM potassium phosphate, monobasic; [B] acetonitrile
gradient: 5 to 45% B in 18 min; 45 to 80% B in 7 min; held at 80% B for 3 min
flow rate: 1.5 mL/min
pressure: 220 bar (3200 psi)
column temp.: 27 °C
detector: UV, 227 nm
injection: 20 μL
sample: 20 μg/mL in water: acetonitrile (86:14)

suitability

application for HPLC

application(s)

vitamins, nutraceuticals, and natural products

Analysis Note

This application demonstrates the suitability of Discovery C18 for the efficient separation of withania standards by the USP monograph.

Legal Information

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany

Analyte

Description
    Withanoside IV
    Withanoside V
    Withaferin A
    12-Deoxywithastramonolide
    Withanolide A
    Withanone
    Withanolide B

analytical column

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Description
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standard

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