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Supelco

Metal Packed GC Column (General Configuration)

phase 3% SP®-1500, matrix 80/120 Carbopack B support, L × O.D. × I.D. 10.0 ft (3.0 m) × 1/8 in. × 2.1 mm

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About This Item

UNSPSC Code:
41115710

material

stainless steel column

parameter

0-230 °C temperature (isothermal or programmed)

technique(s)

gas chromatography (GC): suitable

L × O.D. × I.D.

10.0 ft (3.0 m) × 1/8 in. × 2.1 mm

matrix

80/120 Carbopack B support

matrix active group

3% SP®-1500 phase

column type

packed GC

compatibility

for use with General Configuration

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General description

Packed column made up of stainless steel are basically employed when higher carrier gas flow rates are employed as they can sustain elevated pressure. Stainless steel columns are usually washed with dilute hydrochloric acid, then with water followed by methanol, acetone, methylene dichloride and n-hexane in order to remove any corrosion products and traces of lubricating agents.
An economical metal column should be used for less demanding applications where the inertness of glass is not required. This column is of a general configuration, and can be carefully bent to fit most instruments.

Application

Carbopack B SP-1500 packed gas chromatographic column maybe used for the analysis of toluene in order to study the activity and three dimensional distribution of toluene degrading Pseudomonas putida in a multispecies biofilm assessed by quantitative in situ hybridization and scanning confocal laser microscopy.

Legal Information

SP is a registered trademark of Sigma-Aldrich Co. LLC

Storage Class Code

11 - Combustible Solids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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S Møller et al.
Applied and environmental microbiology, 62(12), 4632-4640 (1996-12-01)
As a representative member of the toluene-degrading population in a biofilter for waste gas treatment, Pseudomonas putida was investigated with a 16S rRNA targeting probe. The three-dimensional distribution of P. putida was visualized in the biofilm matrix by scanning confocal
Introduction to Analytical Gas Chromatography.
Scott RPW.
Science, 102-108 (1997)

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