ZRB1755

Anti-P2X7 Antibody, clone 1D23-1 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonym(s): ATP receptor, P2X purinoceptor 7, P2Z receptor, Purinergic receptor
• UNSPSC Code: 12352203
• NACRES: NA.43
• conjugate: unconjugated
• application: ELISA; ICC; IHC; WB; affinity binding assay
• clone: 1D23-1, recombinant monoclonal
• species reactivity: human
• technique(s): ELISA: suitable; affinity binding assay: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable

Properties

• biological source: rabbit
• Quality Level: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 1D23-1, recombinant monoclonal
• description: recombinant, expressed in HEK 293 cells
• product line: ZooMAb^® learn more
• form: lyophilized
• purified by: using Protein A
• species reactivity: human
• packaging: antibody small pack of 25 μL
• greener alternative product characteristics: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• technique(s): ELISA: suitable; affinity binding assay: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• isotype: IgG
• epitope sequence: C-terminal
• Protein ID accession no.: NP_002553
• UniProt accession no.: Q99572
• shipped in: ambient
• storage temp.: 2-8°C

Description

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 1D23-1 is a ZooMAb^® rabbit recombinant monoclonal antibody that specifically detects P2X purinoceptor 7 (P2X7). It targets an epitope within 23 amino acids from the C-terminal region.

Immunogen

KLH-conjugated linear peptide corresponding to 23 amino acids from the C-terminal region of human P2X purinoceptor 7 (P2X7).

Application

Quality Control Testing

Evaluated by Western Blotting in Human brain tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected P2X7 in Human brain tissue lysate.

Tested Applications

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected P2X7 in SK-MEL-28 cells.

Enzyme Immunoassay (ELISA) Analysis: A serial of dilution from a representative lot detected GST-tagged full-length Human P2X7.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected P2X7 in Human cerebral cortex tissue sections.

Affinity Binding Assay: A representative lot of this antibody bound P2X7 peptide with a KD of 2.0 x 10-9 in an affinity binding assay.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

P2X purinoceptor 7 (UniProt: Q99572; also known as P2RX7, ATP receptor, P2Z receptor, Purinergic receptor) is encoded by the P2RX7 gene (Gene ID: 5027) in human. P2RX7 is a multi-pass membrane protein of the P2X receptor family that is widely expressed with highest levels reported in brain and immune tissues. It contains two cytoplasmic domains (aa 1-25; 356-595); two transmembrane domains (aa 26-46; 335-355); and an extracellular domain (aa 47-334). Eight different isoforms of P2RX7 have been described that are generated via alternative splicing. Functional P2RX7 are shown to be organized as homomeric and as heteromeric trimers.  They ACT^® as receptor for ATP and serve as a ligand-gated iOn^™ channel. They are also responsible for ATP-dependent lysis of macrophages through the formation of membrane pores that are permeable to large molecules. They can function in both FAST synaptic transmission and in ATP-mediated lysis of antigen-presenting cells. P2RX7 can undergo phosphorylation that results in its inactivation.  ADP-ribosylation at arginine 125 is shown to be essential for its channel gating activity. Also, palmitoylation of several cysteines in the C-terminal cytoplasmic tail is required for efficient localization to cell surface. In the absence of ATP, it can function as a scavenger receptor in the recognition and engulfment of apoptotic cells. P2X7 receptor is dephosphorylated at tyrosine 343 upon activation, which strongly inhibits the membrane current and retards membrane blebbing. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, Affinity^™, reproducibility, and stability over conventional monoclonals.(Ref.: Gu, BJ., et al. (2011). J. Immunol. 187(5); 2365-2375; Kim, M., et al. (2001). EMBO J. 20(22); 6347-6358).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitution

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

ACT is a registered trademark of Merck KGaA, Darmstadt, Germany

Affinity is a trademark of Mine Safety Appliances Co.

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

iOn is a trademark of 3DXTech

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable