SRP5339

PDE1A, active, His tagged human

recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

• Synonym(s): HCAM1, HSPDE1A, MGC26303
• UNSPSC Code: 12352200
• NACRES: NA.32

Properties

• recombinant: expressed in baculovirus infected Sf9 cells
• Assay: ≥70% (SDS-PAGE)
• form: buffered aqueous glycerol solution
• specific activity: 10412-14134 nmol/min·mg
• mol wt: ~64 kDa
• NCBI accession no.: NM_005019
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... PDE1A(5136)

Description

General description

PDE1A is a member of the cyclic nucleotide phosphodiesterase family that is stimulated by Ca2+-calmodulin. PDE1A is a cGMP-specific phosphodiesterase and hydrolyzes cGMP to 5′-GMP. Calmodulin is tightly bound to PDE1A in human spermatozoa and this results in permanent activation of PDE1A. Inhibition of PDE1A function significantly attenuates vascular smooth muscle cell growth by decreasing proliferation via G1 arrest and induces apoptosis. In vascular smooth muscle cell, PDE1A is important in regulating growth and survival of these cells and thus can contribute to the neointima formation in atherosclerosis and restenosis.

Physical form

Supplied in 50 mM sodium phosphate, pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 0.2 mM DTT, 25% glycerol.

Preparation Note

After opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles.

Safety Information

• Pictograms: GHS08,GHS07
• Signal Word: Danger
• Hazard Statements: H315,H319,H360D
• Precautionary Statements: P202 - P264 - P280 - P302 + P352 - P305 + P351 + P338 - P308 + P313
• Hazard Classifications: Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2
• Storage Class Code: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable