SRP5012
CDK9/CyclinK, active, GST tagged human
PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution
Synonym(s):
C-2k, CCNK, CDC2L4, CPR4, MGC9113, PITALRE, TAK
Sign Into View Organizational & Contract Pricing
All Photos(2)
About This Item
UNSPSC Code:
12352200
NACRES:
NA.32
recombinant
expressed in baculovirus infected Sf9 cells
product line
PRECISIO® Kinase
Assay
≥70% (SDS-PAGE)
form
buffered aqueous glycerol solution
specific activity
15-21 nmol/min·mg
mol wt
~67 kDa (CyclinK)
~68 kDa (CDK9)
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... CCNK(8812) , CDK9(1025)
General description
CDK9/CyclinK, active, GST tagged human comprises recombinant full-length human CDK9 and cyclinK proteins co-expressedin Sf9 insect cells with an N-terminal GST tag. Cyclin-dependent protein kinase 9 (CDK9)/CyclinK is a member of the cyclin-dependent protein kinase (CDK) family. CDK9 is a component of the multiprotein complex mitogen-activated protein kinase 7 and transcription elongation factor β (TAK/P-TEFβ).
Biochem/physiol Actions
CDKs are closely related to yeast CDk1, cdc28 and cdc2 and arean important regulators of the cell cycle. CDK9 can modulate RNA polymerase II-directed transcription by phosphorylating the C-terminal domain of the largest subunit of RNA polymerase II. CDK9 forms a complex with and is regulated by its regulatory subunit cyclin T or cyclin K. CDK9 also interacts with the human immune deficiency virus 1 (HIV-1) transactivating regulatory protein (Tat) protein, which suggests a possible involvement of this protein in AIDS.
Physical form
Supplied in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Preparation Note
after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles
Legal Information
PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Ranadip Mandal et al.
Cellular and molecular life sciences : CMLS, 79(12), 597-597 (2022-11-19)
Cervical cancer is the fourth most frequently diagnosed and fatal gynecological cancer. 15-61% of all cases metastasize and develop chemoresistance, reducing the 5-year survival of cervical cancer patients to as low as 17%. Therefore, unraveling the mechanisms contributing to metastasis
Phosphorylation of the RNA polymerase II carboxyl-terminal domain by CDK9 is directly responsible for human immunodeficiency virus type 1 Tat-activated transcriptional elongation
Kim YK, et al.
Molecular and Cellular Biology, 22(13), 4622-4637 (2002)
The 7SK small nuclear RNA inhibits the CDK9/cyclin T1 kinase to control transcription
Yang Z, et al.
Nature, 414(6861), 317-322 (2001)
Z Yang et al.
Nature, 414(6861), 317-322 (2001-11-20)
The human positive transcription elongation factor P-TEFb, consisting of a CDK9/cyclin T1 heterodimer, functions as both a general and an HIV-1 Tat-specific transcription factor. P-TEFb activates transcription by phosphorylating RNA polymerase (Pol) II, leading to the formation of processive elongation
F Bullrich et al.
Cancer research, 55(6), 1199-1205 (1995-03-15)
Orderly progression through the cell cycle requires sequential activation and inactivation of cyclin-dependent kinases (cdks). This is achieved in part through the association of cdks with positive regulators called cyclins and inactivation of cyclin-cdk complexes by a rapidly growing number
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service