NA0500
GenElute™ HP Plasmid Megaprep Kit
sufficient for 5 preparations
Synonym(s):
Gen Elute, GenElute™ HP Plasmid Kit
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About This Item
UNSPSC Code:
12352200
NACRES:
NA.52
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usage
sufficient for 5 preparations
greener alternative product characteristics
Inherently Safer Chemistry for Accident Prevention
Learn more about the Principles of Green Chemistry.
General description
The GenElute HP Plasmid Megaprep Kit offers a simple, rapid, and cost-effective method for isolating plasmid DNA from recombinant E. coli cultures. This high performance kit features vacuum filter units for rapid lysate clearing and for DNA-silica binding. Up to 5 mg of high copy plasmid DNA can be recovered from 200 mL-1.0 L LB medium or 200-600 mL TB medium of E. coli culture in less than 1.5 hours. The actual yield depends on the strain, the plasmid, and the culture medium used.
An overnight recombinant E. coli culture is harvested with centrifugation and subjected to a modified alkaline-SDS lysis procedure followed by adsorption of the DNA onto a silica membrane in the presence of high salts. Contaminants are removed by two wash steps. Finally, the bound DNA is then eluted in Elution Solution (Tris-HCl) or water.
An overnight recombinant E. coli culture is harvested with centrifugation and subjected to a modified alkaline-SDS lysis procedure followed by adsorption of the DNA onto a silica membrane in the presence of high salts. Contaminants are removed by two wash steps. Finally, the bound DNA is then eluted in Elution Solution (Tris-HCl) or water.
The GenElute HP Plasmid Megaprep Kit offers a simple, rapid, and cost-effective method for isolating plasmid DNA from recombinant E. coli cultures. This high performance kit features vacuum filter units for rapid lysate clearing and for DNA-silica binding. Up to 5 mg of high copy plasmid DNA can be recovered from 200 mL-1.0 L LB medium or 200-600 mL TB medium of E. coli culture in less than 1.5 hours. The actual yield depends on the strain, the plasmid, and the culture medium used.
An overnight recombinant E. coli culture is harvested with centrifugation and subjected to a modified alkaline-SDS lysis procedure followed by adsorption of the DNA onto a silica membrane in the presence of high salts. Contaminants are removed by two wash steps. Finally, the bound DNA is then eluted in Elution Solution (Tris-HCl) or water.
The DNA is ready for immediate use in applications such as restriction digest, cloning, sequencing, PCR, and transfection.
An overnight recombinant E. coli culture is harvested with centrifugation and subjected to a modified alkaline-SDS lysis procedure followed by adsorption of the DNA onto a silica membrane in the presence of high salts. Contaminants are removed by two wash steps. Finally, the bound DNA is then eluted in Elution Solution (Tris-HCl) or water.
The DNA is ready for immediate use in applications such as restriction digest, cloning, sequencing, PCR, and transfection.
Application
GenElute™ HP Plasmid Megaprep Kit has been used to purify plasmids.
The recovered plasmid DNA is predominately in its supercoiled form. Genomic DNA and RNA are below detectable levels by ethidium bromide stained agarose gel electrophoresis. The DNA is ready for immediate use in downstream applications such as restriction digestion, ligation, sequencing, PCR, transformation, and transfection.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.
Features and Benefits
- From harvested bacterial culture to pure plasmid DNA in 90 minutes or less
- Up to 5 mg of high-copy plasmid DNA
- Efficient vacuum format
- No phenol/chloroform extraction of alcohol precipitation required
Legal Information
GenElute is a trademark of Sigma-Aldrich Co. LLC
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Resp. Sens. 1 - Skin Corr. 1B - STOT RE 1 Inhalation - STOT SE 3
Target Organs
Central nervous system, Lungs
Storage Class Code
3 - Flammable liquids
Flash Point(F)
77.0 °F - closed cup
Flash Point(C)
25 °C - closed cup
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