N6789

Anti-NUCB2 (C-terminal) antibody produced in rabbit

~1.5 mg/mL, affinity isolated antibody, buffered aqueous solution

• Synonym(s): Anti-NEFA, Anti-Nef, Anti-Nesfatin, Anti-Nucleobindin 2, Anti-P54
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 200
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~54 kDa
• species reactivity: rat, mouse
• packaging: antibody small pack of 25 μL
• concentration: ~1.5 mg/mL
• technique(s): indirect immunofluorescence: 10-20 μg/mL using NIH3T3 cells; western blot: 1.5-3.0 μg/mL using rat adrenal extract (S1 fraction) and mouse testis extract (S1 fraction)
• UniProt accession no.: Q9JI85
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: rat ... Nucb2(59295)

Description

General description

Nucleobindin 2 (NUCB2), also known as DNA binding/EF-hand/acidic protein (NEFA), p54, is encoded by the gene mapped to human chromosome 11p15. It belongs to the nucleobindin family of EF-hand Ca²⁺ - binding proteins. NUCB2 contains multiple functional domains, including a Leu/Ile rich region, a putative nuclear localization signal, a DNA binding domain, two Ca²⁺ -binding EF-hand motifs and a leucine zipper region. NUCB2 is widely distributed and is localized to the Golgi apparatus. NUCB2 has been shown to interact with both necdin and ARTS-1, through the EF-hand motif.

Specificity

Anti-NUCB2 (C-terminal) specifically recognizes rat and mouse NUCB2.

Application

Anti-NUCB2 (C-terminal) antibody produced in rabbit has been used in immunoblotting and immunofluorescence.

Biochem/physiol Actions

Nucleobindin 2 (NUCB2) has been implicated in various functions, including Ca²⁺ homeostasis, hypothalamic regulation of feeding and tumor necrosis factor (TNF)-receptor-1 (TNFR1)shedding. The NUCB2-ARTS-1 complex is essential for the proteolytic cleavage and release of TNFR1 to the extracellular compartment. NUCB2 has been identified as a novel satiety molecule in the hypothalamus associated with melanocortin signaling in the central nervous system. NUCB2/nesfatin-1 has been shown to induce suppression of food-intake in rats.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

Store at –20 °C. For continuous use, the product may be stored at 2–8 °C for up to one month. For extended storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable