MAK479

NADP/NADPH Assay Kit

sufficient for 100 colorimetric tests

• UNSPSC Code: 12161503
• NACRES: NA.84

Properties

• Quality Level: 100
• detection method: colorimetric
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

Pyridine nucleotides play an important role in metabolism and, thus, there is continual interest in monitoring their concentration levels. Quantitative determination of NADP+ /NADPH has applications in research pertaining to energy transformation and redox state of cells or tissue.

Features and Benefits

• The linear detection range of the kit is 0.1 µM to 10 µM NADP+ /NADPH
• Compatible with high-throughput handling systems.
• Convenient, the procedure involves adding a single working reagent, and reading the optical density at time zero and 30 minutes at room temperature.

Suitability

The kit is suitable for detection of NADP+ /NADPH concentrations and ratios in cell or tissue extracts.

Principle

The NADP/NADPH Assay Kit is based on a glucose dehydrogenase cycling reaction, in which the formed NADPH reduces a formazan (MTT) reagent. The intensity of the reduced product color, measured at 565 nm, is proportionate to the NADP+ /NADPH concentration in the sample. This assay is highly specific for NADP+ /NADPH and is not interfered by NAD+ /NADH.

Safety Information

• Pictograms: GHS02,GHS05,GHS07
• Signal Word: Danger
• Hazard Statements: H225,H290,H315,H319,H336
• Precautionary Statements: P210 - P233 - P234 - P240 - P303 + P361 + P353 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Flam. Liq. 2 - Met. Corr. 1 - Skin Irrit. 2 - STOT SE 3
• Target Organs: Respiratory system
• Storage Class Code: 3 - Flammable liquids
• WGK: WGK 2