The Carbonic Anhydrase (CA) Activity Assay Kit (Colorimetric) has not been tested with bacteria samples, so there are no specific recommendations available for this application. Additionally, this kit has not been tested with mammalian lysates. The lysis buffer required for the assay may be incompatible with the buffers provided, and the substrate may not be compatible or may cross-react with other biological components found in a lysate.
MAK404
Carbonic Anhydrase (CA) Activity Assay Kit (Colorimetric)
sufficient for 100 colorimetric tests
Synonym(s):
CA Enzyme Activity Assay Kit (Colorimetric)
Sign Into View Organizational & Contract Pricing
All Photos(1)
About This Item
UNSPSC Code:
12161503
Recommended Products
detection method
colorimetric
relevant disease(s)
endocrinological disorders, diabetes; cancer; hematological disorder
storage temp.
−20°C
General description
Carbonic anhydrases (CA) are zinc enzymes present in both prokaryotes and eukaryotes. They catalyze efficiently the reversible hydration of CO2 to bicarbonate. Their important patho-physiological roles in respiration, pH and CO2 homeostasis, secretion, gluconeogenesis, ureagenesis etc. make it an important drug target. Characteristic increase and decrease of CA Activity is observed in different physiological conditions and diseases such as anemia, Thalassemia, Hypothyroidism, Hyperthyroidism, and in several cases of lung and liver diseases and Leukemia.
Features and Benefits
Carbonic Anhydrase (CA) Activity Assay Kit:
- Compatible with high-throughput handling systems.
Suitability
The kit is suitable for the detection of carbonic anhydrase activity in hemolysates and serum, as well as the determination of enzymatic activity of purified CA.
Principle
The assay utilizes the esterase activity of an active CA on an ester substrate which releases a chromophore.The released product can be easily quantified using a spectrophotometric multi-well plate reader. In the presence of a CA specific inhibitor, the enzyme loses its activity which results in a decrease of absorbance.To measure specific CA activity, the CA inhibitor Acetazolamide is included.Acetazolamide is a potent inhibitor of CA I, CA II, CA IV, CA IX, CA XII etc. except CA III.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Dam. 1 - Resp. Sens. 1 - Skin Irrit. 2
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Choose from one of the most recent versions:
Certificates of Analysis (COA)
Lot/Batch Number
Don't see the Right Version?
If you require a particular version, you can look up a specific certificate by the Lot or Batch number.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
-
Is MAK404-1KT Carbonic Anhydrase Activity Assay Kit (Colorimetric) suitable for use with bacteria and mammalian tissue lysates?
1 answer-
Helpful?
-
Active Filters
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service
