M2154

Medium 199

With Earle′s salts and sodium bicarbonate, without ʟ-glutamine, sterile liquid, suitable for cell culture

• Synonym(s): M199 Medium, TCM 199
• UNSPSC Code: 12352207
• NACRES: NA.75

Properties

• product name: Medium 199, With Earle′s salts and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture
• sterility: sterile-filtered
• form: liquid
• technique(s): cell culture | mammalian: suitable
• impurities: endotoxin, tested
• pH: 7.0-7.6 (25 °C, 209 g/L)
• components: sodium pyruvate: no; L-glutamine: no; NaHCO₃: yes; HEPES: no; phenol red: yes; Earle’s salts (5% CO₂): yes
• shipped in: ambient
• storage temp.: 2-8°C

Description

General description

Medium 199 is a combination of vitamins, amino acids, and other factors that were originally developed as a completely defined media formulation for the culture of primary explants. This medium, when properly supplemented has broad applicability, particularly for non-transformed cells. Medium 199 is widely used for vaccine production, in vitro cultivation of primary pancreatic explants, and lens tissues.

Application

Medium 199 has been used:

• to culture rumen epithelial cells from yearling Speckle Park beef heifers
• as a component of the maturation medium for the incubation of Cumulus oocyte complexes (COCs) from bovine ovaries
• as a component of the Dulbecco′s modified Eagle medium (DMEM) mixture to maintain rat neonatal cardiac myocytes (NCM)

Reconstitution

Supplement with 0.1 g/L L-glutamine.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable