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M0671

Supelco

Recombinant Molecular Weight Marker

mol wt 15,000-150,000 Da

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About This Item

UNSPSC Code:
12352200

usage

1 vial sufficient for 100 uses (10 × 10 cm mini gels)
1 vial sufficient for 50 uses (large format 16 × 18 cm gels)

Quality Level

mol wt

15,000-150,000 Da

storage temp.

2-8°C

Application

Recombinant Molecular Weight Marker has been used for determining the size of protein bands in western blot and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE).

Other Notes

The recombinant molecular weight standard for SDS-PAGE contains seven precisely sized proteins, M.W. 15, 25, 35, 50, 75, 100 and 150 kDa. Recombinant proteins produce sharp bands for precise size determination since they are not glycosylated.

Physical form

Solution containing approx. 0.8 mg/ml total protein in 125 mM Tris-HCl, pH 6.8, 2% SDS, 10% glycerol, 200 mM 2-mercaptoethanol, 0.007% bromphenol blue.

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Eye Irrit. 2 - Skin Sens. 1

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Biochemical and Biophysical Research Communications, 369, 1114-1119 (2008)
Eduardo L V Silveira et al.
Clinical and vaccine immunology : CVI, 15(8), 1292-1300 (2008-06-27)
Earlier studies have demonstrated in A/Sn mice highly susceptible to Chagas' disease protective immunity against lethal Trypanosoma cruzi infection elicited by vaccination with an open reading frame (ORF) expressed by amastigotes. In our experiments, we used this mouse model to

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