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I8896

Sigma-Aldrich

IGEPAL® CA-630

for molecular biology

Synonym(s):

Octylphenoxy poly(ethyleneoxy)ethanol, branched

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$174.00
100 ML
$298.00

$174.00

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50 ML
$174.00
100 ML
$298.00

About This Item

Linear Formula:
(C2H4O)nC14H22O
CAS Number:
9002-93-1
UNSPSC Code:
12352104
NACRES:
NA.31

$174.00

In StockDetails

Request a Bulk Order

grade

for molecular biology

Quality Level

200

description

non-ionic

form

liquid

technique(s)

IR spectroscopy: suitable

CMC

0.08 mM (20-25°C)

HLB

13

foreign activity

DNase and RNase, none detected

SMILES string

O(CCO)c1ccc(cc1)C(CC(C)(C)C)(C)C

InChI

1S/C16H26O2/c1-15(2,3)12-16(4,5)13-6-8-14(9-7-13)18-11-10-17/h6-9,17H,10-12H2,1-5H3

InChI key

JYCQQPHGFMYQCF-UHFFFAOYSA-N

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General description

IGEPAL® CA-630 is a nonionic, non-denaturing detergent suitable for solubilization, isolation and purification of membrane protein complexes.It is chemically indistinguishable from Nonidet P-40.[1]

Application

IGEPAL® CA-630 has been used
  • as neutral detergent in homogenizing buffer during western blotting of mouse muscle homogenates[2]
  • In X-gal staining for studying the lacZ expression in E8.5-E10.5 embryos[3]
  • In Cell-Lysis (CL) buffer for preparing RT-qPCR-ready cell lysates[4]

Other Notes

Nonionic, non-denaturing detergent

Quality

Chemically indistinguishable from Nonidet P-40, which is no longer commercially available.

Caution

May develop turbidity or sediment during storage. A clear liquid can be obtained on heating to 40°C.

Legal Information

IGEPAL is a registered trademark of Solvay

Signal Word

Danger

Hazard Statements

H302,H315,H318,H410

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Irrit. 2

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

483.8 °F - closed cup

Flash Point(C)

251 °C - closed cup

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
MKCW7585
MKCV4219
MKCR5566
MKCR3234
MKCS4114

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Joseph A Roche et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 59(11), 964-975 (2011-11-02)
Mutations in the DYSF gene that severely reduce the levels of the protein dysferlin are implicated in muscle-wasting syndromes known as dysferlinopathies. Although studies of its function in skeletal muscle have focused on its potential role in repairing the plasma
Bojana Lucic et al.
Nature communications, 10(1), 4059-4059 (2019-09-08)
HIV-1 recurrently targets active genes and integrates in the proximity of the nuclear pore compartment in CD4+ T cells. However, the genomic features of these genes and the relevance of their transcriptional activity for HIV-1 integration have so far remained
Amina Abbadi et al.
The Journal of biological chemistry, 291(3), 1448-1455 (2015-11-26)
Many cells, including murine airway epithelial cells, respond to a variety of inflammatory stimuli by synthesizing leukocyte-adhesive hyaluronan (HA) cables that remain attached to their cell surfaces. This study shows that air-liquid interface cultures of murine airway epithelial cells (AECs)
Per Wasteson et al.
Development (Cambridge, England), 135(10), 1823-1832 (2008-04-18)
Aortic smooth muscle cells (SMCs) have been proposed to derive from lateral plate mesoderm. It has further been suggested that induction of SMC differentiation is confined to the ventral side of the aorta, and that SMCs later migrate to the
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Genome-wide association studies (GWAS) have identified hundreds of loci associated with coronary artery disease (CAD) and blood pressure (BP) or hypertension. Many of these loci are not linked to traditional risk factors, nor do they include obvious candidate genes, complicating
View All Related Papers

Protocols

Protocol for Anti Ago-RNA Immunoprecipitation from Mammalian Cells Using the RIP Kit

Procedure and protocol for Anti Ago-RNA Immunoprecipitation from mammalian cells using the RIP kit

Pull-down assays

This page provides information about different pull-down assays for the further isolation of multiprotein complexes to identify their components with products from Cytiva.

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Investigate in vitro protein-protein interactions with pull-down assays, utilizing affinity, GST pull-down, TAP, and co-immunoprecipitation methods.

Questions

1–10 of 17 Questions  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  3. In which solvent(s) is Atazanavir Sulfate soluble?

    1 answer

    1. Atazanavir Sulfate is soluble in DMSO: 104mg/mL and EtOH: 20mg/mL.

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  4. What is the storage temperature for this item?

    1 answer

    1. This product may be stored at room temperature. For products with no specified storage temperature given on the label or product page, ambient storage may be assumed. Products that require controlled temperature storage, such as refrigeration or freezer conditions, storage temperatures are published on the product label, Certificate of Analysis, the Safety Data Sheet (SDS).

      Helpful?

  5. Hi, I am enquiring about the storage temperature of product IGEPAL(R) CA-630 (I8896-100ml). The storage class is listed in the SDS but no temperature upper and lower limits. Would I be able to request this information. Thank you

    1 answer

    1. This product may be stored at room temperature. For products with no specified storage temperature given on the label or product page, ambient storage may be assumed. Products that require controlled temperature storage, such as refrigeration or freezer conditions, storage temperatures are published on the product label, Certificate of Analysis, and the Safety Data Sheet (SDS).

      Helpful?

  6. What is the molecular weight of Product No. I8896, IGEPAL® CA-630?

    1 answer

    1. The approximate molecular weight of this product is 603 Daltons. The average EO (ethylene oxide) units per molecule are 9.

      Helpful?

  7. What is the wt/vol % of this IGEPAL reagent? I want to use it for ATAC-seq and in the protocols people always mention the concentration of the stock is 10% wt/vol but I can't find this information on the specifications of this product. Thanks

    1 answer

    1. This product is provided as a neat liquid with a concentration of 100% which can be further diluted. The density of IGEPAL CA-630 is 1.06 g/cm3 at 25 °C. For a 10% wt./vol. solution 9.43 mL IGEPAL is needed per 100 mL of solution.

      The product is generally stable based on the chemical composition and a 10% (w/v) aqueous solution may be stable refrigerated for at least one month. However, it is important to minimize the exposure of the solution to oxygen (keep closed soon after use). The product does have a tendency to oxidize in air forming peroxides through a free radical process. In addition, some bacterial growth may occur which is evidenced by an odor or formation of a precipitate. Preservatives added to the solution may prevent bacterial growth. Please view the data sheet below: https://www.braskem.com.br/Portal/Principal/Arquivos/html/boletm_tecnico/PP%20Chemical%20Resistance.pdf

      Helpful?

  8. What is the formula weight of Product No. I8896, IGEPAL® CA-630?

    1 answer

    1. The formula weight of this product is 603.

      Helpful?

  9. What does "Molecular Biology Grade" mean?

    1 answer

    1. Molecular Biology Grade means that this product must meet the DNase, RNase, and Nickase specification on the Certificate of Analysis for each lot of the product.

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  10. What is the CMC (critical micellar concentration) value of Product No. I8896, IGEPAL® CA-630?

    1 answer

    1. The CMC value of this product is 0.083 mM.

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1–10 of 17 Questions  

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