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H6024

Sigma-Aldrich

Hoechst Stain solution

Synonym(s):

Bisbenzimide H 33258

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About This Item

CAS Number:
23491-45-4
MDL number:
MFCD00012679
UNSPSC Code:
12352207
PubChem Substance ID:
24895727

form

solution

application(s)

histology

storage temp.

2-8°C

SMILES string

O.Cl.Cl.Cl.CN1CCN(CC1)c2ccc3NC(=NCc3c2)c4ccc5NC(=NCc5c4)c6ccc(O)cc6

InChI

1S/C27H28N6O.3ClH.H2O/c1-32-10-12-33(13-11-32)22-5-9-25-21(15-22)17-29-27(31-25)19-4-8-24-20(14-19)16-28-26(30-24)18-2-6-23(34)7-3-18;;;;/h2-9,14-15,34H,10-13,16-17H2,1H3,(H,28,30)(H,29,31);3*1H;1H2

InChI key

OWRSPPSBNWJJAR-UHFFFAOYSA-N

General description

Hoechst stains are part of a family of blue fluorescent dyes commonly used to stain DNA. It gets incorporated biosynthetically into interphase nuclei in fixed cytologic preparations and in unfixed cultured cells. It can also be used to detect biosynthetic incorporation of 5-bromodeoxyuridine into the DNA of living cells. Hoechst stains property may allow fluorescence-activated cell and chromosome sorting according to the extent of DNA synthesis which may provide a bridge between biochemical and cytologic analyses of processes related to DNA replication.

Application

A fluorescent DNA stain qualified for use in Mycoplama staining.

Components

Contains 0.5μg/ml Hoechst bisbenzimide 33258 fluorochrome stain and thimerosal.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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S A Latt et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 23(7), 493-505 (1975-07-01)
A number of applications of the detection of deoxyribonucleic acid synthesis by fluorescence microscopy are illustrated. These include (a) the analysis of sister chromatid exchanges and sister chromatid segregation at mitosis, (b) the location of chromosome regions containing deoxyribonucleic acid
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