G6048
Galactokinase human
recombinant, expressed in E. coli
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About This Item
Recommended Products
recombinant
expressed in E. coli
Quality Level
form
solution
specific activity
≥1400 unit/μg protein
mol wt
42 kDa
shipped in
dry ice
storage temp.
−70°C
Biochem/physiol Actions
Galactokinase catalyzes the phosphorylation of αD-galactose to produce galactose-1-phosphate as part of the Leloir pathway.
Physical properties
N-terminal GST-tagged 42 kDa full length protein
Unit Definition
One unit will convert 1.0 picomole of galactose to galactose-1-phosphate per minute at pH 7.4 at 30 °C.
Physical form
Supplied as a solution in 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 20% glycerol, 3 mM DTT and 10-250 mM imidazole.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2
Storage Class Code
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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Dariusz Abramczyk et al.
Eukaryotic cell, 11(3), 334-342 (2012-01-03)
The regulation of the Saccharomyces cerevisiae GAL genes in response to galactose as a source of carbon has served as a paradigm for eukaryotic transcriptional control over the last 50 years. Three proteins--a transcriptional activator (Gal4p), an inhibitor (Gal80p), and
Shivani Malik et al.
Nucleic acids research, 40(8), 3348-3363 (2011-12-27)
Recently, we have demonstrated a predominant association of Rad26p with the coding sequences but not promoters of several GAL genes following transcriptional induction. Here, we show that the occupancy of histone H2A-H2B dimer at the coding sequences of these genes
Aurélie Egert et al.
Plant & cell physiology, 53(5), 921-929 (2012-03-23)
Galactokinase (GALK, EC 2.7.1.6) is a cytosolic enzyme with a wide occurrence across the taxonomic kingdoms. It catalyzes the phosphorylation of α-d-galactose (Gal) to α-d-Gal-1-P. The cytotoxicity of free (unphosphorylated) Gal is well documented in plants and causes marked defects.
Nils Janzen et al.
Archives of medical research, 42(7), 608-612 (2011-12-14)
Galactokinase (GALK) deficiency is an autosomal recessive disorder causing cataract formation that can be prevented or mitigated by early diagnosis and galactose-restricted diet. The aim of this retrospective study was to explore whether GALK-deficiency meets the criteria for neonatal mass
Lei Li et al.
Carbohydrate research, 355, 35-39 (2012-05-29)
Galactokinase (GalK), particularly GalK from Escherichia coli, has been widely employed for the synthesis of sugar-1-phosphates. In this study, a GalK from Bifidobacterium infantis ATCC 15697 (BiGalK) was cloned and over-expressed with a yield of over 80 mg/L cell cultures.
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