E9902

Ephrin-A1/Fc Chimera from mouse

>90% (SDS-PAGE), recombinant, expressed in NSO cells, lyophilized powder

• Synonym(s): B61, EFL-1, LERK-1
• UNSPSC Code: 51111800
• NACRES: NA.32
• MDL number: MFCD01867530

Properties

• biological source: mouse
• Quality Segment: 200
• recombinant: expressed in NSO cells
• assay: >90% (SDS-PAGE)
• form: lyophilized powder
• potency: 0.16-10 ng/mL
• mol wt: monomer calculated mol wt 46.8 kDa, 50-55 kDa by SDS-PAGE (reducing)
• packaging: pkg of 200 μg
• storage condition: avoid repeated freeze/thaw cycles
• impurities: endotoxin, tested
• UniProt accession no.: P20827
• storage temp.: −20°C
• Gene Information: mouse ... Efna1(13636)

Description

Biochem/physiol Actions

EphA1 (ephrin receptor A1) plays an essential role in development, and is involved in the guidance of axonal and cell migration in nervous system.[1] Disruption of ephrin signaling might be involved in the tumorigenesis of clear cell renal cell carcinoma, and absence of EphA1 has a favorable prognosis in the same.[2] It might also be involved in the tumorigenesis and progression of prostate cancer, and might have potential as a marker for prognosis, as well as a therapeutic target.[3] Studies show that it reduces the risk of Alzheimer′s disease, by hindering pathological changes in brain areas such as, hippocampus, lateral occipitotemporal and inferior temporal gyri.[4] In esophageal squamous cells, it acts as differentiation marker. Its expression is elevated in esophageal squamous cell carcinoma, and this relates to advanced stage and metastasis to lymph node.[5]

Member of ephrin ligand family that binds Eph receptor tyrosine kinases (EphA1, EphA2, EphA3, EphA4, EphA5, EphA6, EphA7 and EphB1). Involved in pattern formation and morphogenesis. Ephrin-A1 can be detected on fetal osteoblasts, odotoblasts, chondrocytes, and squamous epithelium. Ephrin-A1 is expressed during embryonic vasculogenesis and during tumor-induced angiogenesis.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline.

Analysis Note

The biological activity is measured by its ability to bind recombinant mouse EphA2/Fc in ELISA.

Other Notes

Extracellular domain of mouse ephrin-A1 (amino acids 1-182) fused to the C-terminal 6X histidine-tagged Fc region of human IgG.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable