Derived from a thymic tumour induced in a DBA/2 mouse by methylcholanthrene. The cells were adapted for in vitro growth. The cells have been widely used in toxicity and radiation studies.
Culture Medium
Fischer′s + 2mM Glutamine + 10% Horse Serum (HS)
Subculture Routine
Maintain cultures between 30,000 to 700,000 cells/ml; 5% CO2; 37 C. Cells grow as small clumps.
Other Notes
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