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C2605

Sigma-Aldrich

Cellulase from Aspergillus sp.

greener alternative

aqueous solution

Synonym(s):

Carezyme 4500L®, Carezyme®

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50 ML
$140.00
250 ML
$337.00

$140.00

Estimated to ship onApril 13, 2025

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50 ML
$140.00
250 ML
$337.00

About This Item

CAS Number:
9012-54-8
Enzyme Commission number:
3.2.1.4 (BRENDA, IUBMB)
EC Number:
232-734-4
MDL number:
MFCD00081510
UNSPSC Code:
12352204
NACRES:
NA.54

$140.00

Estimated to ship onApril 13, 2025

Quality Level

200

form

solid

specific activity

≥1000 units/g

greener alternative product characteristics

Waste Prevention
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

greener alternative category

Enabling,

storage temp.

2-8°C

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General description

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in cellulosic ethanol research. For more information see the article in biofiles and Enzymes for Alternative Energy Research

Application

Cellulase from Aspergillus sp. has been used to degrade cello-oligosaccharides into glucose during the analysis of BBC (bioabsorbable bacterial cellulose) biodegradability.[1] The enzyme has also been used in photon correlation spectroscopy to study its effects on unfiltered beer samples. The parameters evaluated in this spectroscopic analysis were the size of suspended particles, flow rate and haze stability.[2]

Biochem/physiol Actions

Cellulases are enzymes that hydrolyze cellulose, a linear polymer of anhydroglucose units linked together by β-1,4-glycosidic bonds, to glucose. Endo-β-D-glucanase is one of the major component enzymes of the cellulase complex. It catalyzes the hydrolysis of cellulose by randomly splitting the sugar residues within the molecule. Exo-β-D-glucanase and β-glucosidase can synergistically convert cellulose into glucose and hence are used on an industrial scale.[3]

Preparation Note

produced by submerged fermentation of a genetically modified Aspergillus microorganism

Other Notes

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Legal Information

A product of Novozyme Corp.
Carezyme is a registered trademark of Novozymes Corp.

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H334

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
0000302577
0000343835
0000343836
0000278645
0000263668

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Monitoring of Beer Filtration Using Photon Correlation Spectroscopy (PCS).
Sensidoni M, et al.
Journal of the Institute of Brewing, 117(4), 639-646 (2011)
Pierre-Louis Bombeck et al.
Biotechnology for biofuels, 10, 293-293 (2017-12-12)
Lignocellulosic biomass will progressively become the main source of carbon for a number of products as the Earth's oil reservoirs disappear. Technology for conversion of wood fiber into bioproducts (wood biorefining) continues to flourish, and access to reliable methods for
Muhammad Sohail et al.
New biotechnology, 25(6), 437-441 (2009-06-26)
Fungal cellulases are well-studied enzymes and are used in various industrial processes. Much of the knowledge of enzymatic depolymerization of cellulosic material has come from Trichoderma cellulase system. Species of Trichoderma can produce substantial amounts of endoglucanase and exoglucanase but
Yang Hu et al.
Journal of biomedical materials research. Part B, Applied biomaterials, 97(1), 114-123 (2011-02-04)
Cellulose biodegradation resulting from enzymolysis generally occurs in nature rather than in the human body because of the absence of cellulose degrading enzymes. In order to achieve in-vivo degradation in human body for in-vivo tissue regeneration applications, we developed a
Akihiko Nakamura et al.
Journal of synchrotron radiation, 20(Pt 6), 859-863 (2013-10-15)
Neutron protein crystallography (NPC) is a powerful tool for determining the hydrogen position and water orientation in proteins, but a much larger protein crystal is needed for NPC than for X-ray crystallography, and thus crystal preparation is a bottleneck. To
View All Related Papers

Related Content

Lysis & Protein Extraction

Cell lysis and protein extraction methods overview various techniques, from detergent solubilization to mechanical disruption, supporting research needs.

Questions

1–9 of 9 Questions  

  1. what is enzyme activity in FPU for  C2605

    1 answer

    1. Unfortunately, the enzyme activity is not tested in terms of filter paper units (FPU). It is presented as U/g.

      Kindly review the following citation that provides a reference to the FPU method (Adney and Baker). As this value is not presented on the certificate of analysis, it may be necessary to re-assess the FPU for every batch received:

      Rodrigues AC, Haven MØ, Lindedam J, Felby C, Gama M. Celluclast and Cellic® CTec2: Saccharification/fermentation of wheat straw, solid-liquid partition and potential of enzyme recycling by alkaline washing. Enzyme Microb Technol. 2015 Nov;79-80:70-7. doi: 10.1016/j.enzmictec.2015.06.019. Epub 2015 Jul 17. PMID: 26320717.

      Helpful?

  2. What is the shelf life of the Cellulase from Aspergillus sp.?

    1 answer

    1. This product is not assigned an expiration date or recommended retest date. Products with no expiration date or recommended retest date should be routinely inspected by customers to ensure they perform as expected. These products are also subject to a one year warranty from the date of shipment. For more information you may access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/life-science/quality-and-regulatory-management/quality-services.

      Helpful?

  3. How are the activity units of Product C2605, Cellulase from Aspergillus sp., listed on the C of A?

    1 answer

    1. The activity of cellulase given on the C of A is in units per gram of liquid.

      Helpful?

  4. What is the density of Product C2605, Cellulase from Aspergillus sp.?

    1 answer

    1. The density of this cellulase solution is approximately 1.2 g/mL.

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  5. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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  6. What are the supplier's working conditions for determining the activity for Product C2605, Cellulase from Aspergillus sp.?

    1 answer

    1. This product is assayed using carboxymethycellulose as substrate, at 40°C, at a pH of 7.5.

      Helpful?

  7. What is the optimum temperature for activity for Cellulase from Aspergillus sp., Product C2605?

    1 answer

    1. At pH 8.5, Novozyme has determined that the optimal activity for this enzyme is ~60°C. The percentage of maximum activity is ~40% of that value at 40°C, and ~80% at 50°C. There is a steep drop in activity above 60°C.

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  8. What is the unit activity of the Cellulase from Aspergillus sp. enzyme, Product C2605?

    1 answer

    1. We provide the unit activity of the product in terms of units/g, for each individual lot. To get a units/mL value, one can use the density value of approximately 1.2 g/mL to convert to a units/mL value for each lot.

      Helpful?

  9. What is the optimum pH for Cellulase from Aspergillus sp., Product C2605?

    1 answer

    1. At a temperature of 40°C, Novozyme has found ~pH 8.0-8.2 to be the optimum pH range for this enzyme.

      Helpful?

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