To prepare 5M solution of Betaine in water, dissolve 29.288 grams of Betaine in 50 ml of nuclease-free distilled or deionized water, and the stock solution should be stored at -20°C. There are no specific requirements for heat or sonication during the preparation process, but the stock solution should be filtered through a 0.22 micron filter before use. Additionally, a stir plate and a stir bar may be used if the powder dissolves slowly.
B0300
Betaine solution
5 M, PCR Reagent
Synonym(s):
TMG, trimethylglycine
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About This Item
Linear Formula:
(CH3)3N+CH2COO−
CAS Number:
Molecular Weight:
117.15
Beilstein:
3537113
MDL number:
UNSPSC Code:
12352200
PubChem Substance ID:
NACRES:
NA.52
Pricing and availability is not currently available.
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grade
PCR Reagent
Quality Level
form
liquid
packaging
vial of 1.5 mL
concentration
5 M
technique(s)
PCR: suitable
color
colorless
application(s)
agriculture
foreign activity
DNase, RNase, none detected
storage temp.
2-8°C
SMILES string
C[N+](C)(C)CC([O-])=O
InChI
1S/C5H11NO2/c1-6(2,3)4-5(7)8/h4H2,1-3H3
InChI key
KWIUHFFTVRNATP-UHFFFAOYSA-N
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General description
Betaine also called trimethylglycine or N,N,N triethylammonium acetate, is an analog of glycine with three methyl groups.[1] It is highly compatible with polymerase chain reaction (PCR) buffer mixture. Betaine is a PCR enhancing reagent that is widely used for improving the yield and specificity of PCR products, especially during the PCR amplification of targets rich in GC content or those that form secondary structures resulting in poor yield. Betaine facilitates DNA strand separation and manages the DNA melting temperature (Tm) difference between the GC and AT pairs in DNA. It stabilizes the ds DNA by equalizing the contribution of GC- and AT-base pairs.[2] Betaine has been broadly used to optimize multiplex and ‘long and accurate′ polymerase chain reaction (LA-PCR). The addition of 1.0-1.7 M aqueous betaine to a PCR mixture has been reported to reduce the base pair composition dependence on DNA strand melting.[3]
Application
Betaine solution has been used as a component of the reaction mixture for:
- loop-mediated isothermal amplification (LAMP) reactions[4]
- polymerase chain reaction for genomic DNA amplification[5]
- quantitative polymerase chain reaction (qPCR) for telomere length analysis[6]
- in the PCR amplification of CGG repeats in the genomic DNA[7]
- reverse-transcription loop-mediated isothermal amplification (RT-LAMP) to detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)[8]
- PCR amplification of DNA-directed RNA polymerase II largest subunit (RPB2)[9]
- reverse transcription during library preparation using Smartseq2[10]
Packaging
1.5mL per vial
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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