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91471

Sigma-Aldrich

Tracy 645 Protein Labeling Kit

suitable for fluorescence

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About This Item

UNSPSC Code:
12352200

fluorescence

λex 638 nm; λem 656 nm in PBS

suitability

suitable for fluorescence

storage temp.

2-8°C

Components

This kit contains sufficient amounts of reactive dye, buffers, and protein purification sets for performing five labeling reactions (1 mg protein each) and for the subsequent purification of the labeled protein.

Other Notes

Tracy 645 is a NIR fluorescent dye that is highly water-soluble and stable. The spectral range above 650 nm is of particular interest in biology analysis and imaging due to the reduced background and improved sensitivity.

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Questions

1–3 of 3 Questions  

  1. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  2. In what buffers can I prepare my protein for labeling with Tracy dye?

    1 answer

    1. We recommend Sodium Bicarbonate buffer, pH 9.5, when working with Tracy  Products 91471 and 75824, but it is necessary to avoid the use of buffers or solvents that contain amine groups, e.g. tris or ammonium ions.

      Helpful?

  3. What ratio of dye to protein for antibodies labled with Tracy Products 91471 and 75824 show the maximum fluorescence intensity?

    1 answer

    1. A dye to protein ratio of 4:1 produces maximum fluorescence intensity.

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