76220
Papain from Carica papaya
powder, ≥3 U/mg
Synonym(s):
Papain powder
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About This Item
Recommended Products
biological source
papaya
form
powder
specific activity
≥3 U/mg
mol wt
Mr ~23000
storage temp.
2-8°C
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Application
Papain is used as a meat tenderizer. It′s also used to break up tissue in the preparation of primary cell cultures.
Biochem/physiol Actions
Papain is a cysteine protease that cleaves peptide bonds and is known to cleave the Fc portion of antibodies.
Unit Definition
1 U corresponds to the amount of enzyme which hydrolyzes 1 μmol N-benzoyl-L-arginine ethyl ester (BAEE, Cat. No. 12880) per minute at pH 6.2 and 25°C
Other Notes
An important cysteine protease ; Peptide synthesis
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
Target Organs
Respiratory system
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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Review: novel cysteine proteases of the papain family.
F Bühling et al.
Advances in experimental medicine and biology, 477, 241-254 (2000-06-13)
M Y Gololobov et al.
Biotechnology and bioengineering, 40(3), 432-436 (1992-07-01)
The published theory of kinetically controlled enzymatic peptide synthesis needed experimental verification. We carried out the measurement of the peptide yield and estimation of the key parameters alpha and beta for papain-catalyzed synthesis of Mal-L-Phe-L-Ala-LLeuNH(2) from Mal-L-Phe-L-AlaOMe and L-LeuNH(2). The
Catalytic mechanism in papain family of cysteine peptidases.
A C Storer et al.
Methods in enzymology, 244, 486-500 (1994-01-01)
Adrienne McMahon
Journal of AOAC International, 99(1), 223-241 (2016-02-10)
The main objective of the AOAC Stakeholder Panel on Infant Formula and Adult Nutritionals (SPIFAN) project is to establish international consensus methods for infant formula and adult nutritionals, which will benefit intermarket supply and dispute resolution. A collaborative study was
Carolina Gomis Perez et al.
Science advances, 8(1), eabl4411-eabl4411 (2022-01-06)
Many cellular activities, such as cell migration, cell division, phagocytosis, and exo-endocytosis, generate and are regulated by membrane tension gradients. Membrane tension gradients drive membrane flows, but there is controversy over how rapidly plasma membrane flow can relax tension gradients.
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