72755
5-(Bromomethyl)fluorescein
BioReagent, suitable for fluorescence, ≥95% (sum of tautomers, GC)
Synonym(s):
5-BMF
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About This Item
Empirical Formula (Hill Notation):
C21H13BrO5
CAS Number:
Molecular Weight:
425.23
UNSPSC Code:
12352200
PubChem Substance ID:
product line
BioReagent
Assay
≥95% (sum of tautomers, GC)
fluorescence
λex 492 nm; λem 515 nm in 0.1 M Tris pH 9.0
suitability
suitable for fluorescence
SMILES string
Oc1ccc2c(Oc3cc(O)ccc3C24OC(=O)c5cc(CBr)ccc45)c1
InChI
1S/C21H13BrO5/c22-10-11-1-4-15-14(7-11)20(25)27-21(15)16-5-2-12(23)8-18(16)26-19-9-13(24)3-6-17(19)21/h1-9,23-24H,10H2
InChI key
OQHKPJAZGYJYTB-UHFFFAOYSA-N
Packaging
Bottomless glass bottle. Contents are inside inserted fused cone.
Other Notes
Labeling of nucleosides and nucleotides at the N-atom; labeling of carboxylic acids: shows very strong absorption and fluorescence.
Storage Class Code
13 - Non Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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P S Mukherjee et al.
Pharmaceutical research, 12(6), 930-936 (1995-06-01)
5-Bromomethyl fluorescein (5-BMF) was evaluated in this work as a pre-column, off-line derivatizing reagent for analytes containing a free carboxyl group. The reagent possessed high molar absorptivity and quantum yield and it's excitation maximum matched the intense 488.0 nm emission
P.S. Mukherjee et al.
Analytical Chemistry, 68, 327-327 (1993)
P S Mukherjee et al.
Analytical chemistry, 68(2), 327-332 (1996-01-15)
This report describes the first application of 5-(bromo-methyl)fluorescein (5-BMF) for the quantitation of a pharmaceutically relevant carboxyl-containing analyte in a biological matrix. An analytical method for quantitation of gamma-(cholesteryloxy)butyric acid (CBA), a relatively new antitumor agent, in different tissues of
Jonathan J Hart et al.
Electrophoresis, 23(1), 81-87 (2002-02-05)
An analytical method for determining thiols and phytochelatins using high-performance capillary electrophoresis coupled with laser-induced fluorescence detection is presented. The technique utilizes the labeling of thiols with the fluorescent reagent 5-bromomethylfluorescein (5-BMF), which is excited by a 488 nm argon
U Alexiev et al.
Proceedings of the National Academy of Sciences of the United States of America, 92(2), 372-376 (1995-01-17)
The pH-indicator dye fluorescein was covalently bound to the surface of the purple membrane at position 72 on the extracellular side of bacteriorhopsin and at positions 101, 105, 160, or 231 on the cytoplasmic side by reacting bromomethylfluorescein with the
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