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MCHT24H48

Millipore

Millicell® Hanging Cell Culture Insert, PET 0.4 μm, 24-well, 48/pk

Discontinued with Replacement Product No. PTHT24H48

Synonym(s):

cell culture inserts, permeable culture insert, plate inserts, tissue culture insert, tissue culture plate

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About This Item

UNSPSC Code:
41121800

material

PET membrane
flat wells
polystyrene housing

sterility

sterile

feature

lid: no

packaging

pack of 48 ea (individually blister packaged)

parameter

50 °C max. temp.

filter diam.

6.5 mm

filtration area

0.3 cm2

size

24 wells

surface area

0.3 cm2

well maximum volume

440 μL

working volume

200 μL

pore size

0.4 μm

input

sample type hematopoietic stem cell(s)
sample type induced pluripotent stem cell(s)
sample type pancreatic stem cell(s)
sample type neural stem cell(s)
sample type cardiac stem cell(s)
sample type: mouse embryonic stem cell(s)
sample type epithelial cells
sample type: human embryonic stem cell(s)
sample type mesenchymal stem cell(s)

binding type

Tissue Culture (TC)-treated surface

General description

Polyethylene Terephthalate (PET) hanging cell culture insert with pore size of 8.0 μm used in a 24-well plate for cell attachment, cell culture, cell differentiation & ICC.Device configuration: 24-well plate, 0.4 μm pore sizeMillicell® cell culture inserts are an easy to use and convenient device for adherent or suspension cells. Allowing the access of media from both their apical and basolateral sides. Cell growth, structure, and function more closely mimic what occurs in vivo. In addition, Millicell® inserts make it possible to study both sides of the cell monolayer.
Millicell® Hanging Inserts: Preloaded in receivers or available standalone
  • For co-culturing and permeability assays
  • Unique design allows easier basolateral access than other hanging inserts with less risk of contamination
  • Available in 5 pore sizes and 3 diameters, including a 1 μm pore size that is optically transparent for better visualization by microscopy
  • Available preloaded in 24-well receiver plates or as individual inserts in 3 diameters

Applications: Cell Attachment, Cell Growth, Cell Differentiation, Immunocytochemistry

Application

Discontinued with replacement product no. PTHT24H48

Legal Information

Millicell is a registered trademark of Merck KGaA, Darmstadt, Germany

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Protocols

Basic Indirect Co-culture on Both Sides of Millicell® Cell Culture Insert Membranes

This page covers the basic indirect co-culture procedure on both sides of Millicell cell culture insert membranes.

Microscopic Examination of Samples

This protocol covers three methods for the microscopic examination of cell samples.

ECM Coating Protocols for Millicell®

This page covers the ECM coating protocols developed for four types of ECMs on Millicell®-CM inserts, Collagen Type 1, Fibronectin, Laminin, and Matrigel.

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