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MABS502

Sigma-Aldrich

Anti-CYP11B1 Antibody, clone 80-7

clone 80-7, from rat

Synonym(s):

Cytochrome P450 11B1, mitochondrial, CYPXIB1, Cytochrome P-450c11, Cytochrome P450C11, Steroid 11-beta-hydroxylase, CYP11B1

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rat

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

80-7, monoclonal

species reactivity

human, rat

technique(s)

immunohistochemistry: suitable
western blot: suitable

isotype

IgG2aκ

NCBI accession no.

NP_000488

UniProt accession no.

P15538

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... CYP11B1(1584)

General description

Cytochrome P450 11B1, mitochondrial (EC1.14.15.4, EC1.14.15.5; UniProt P15538; also known as CYPXIB1, Cytochrome P-450c11, Cytochrome P450C11, Cytochrome P450 subfamily XIB polypeptide 1, Cytochrome P450 XIB1, Steroid 11-beta-monooxygenase, Steroid 11-beta-hydroxylase) is encoded by the CYP11B1 (Also known as CPN1, CYP11B, FHI, P450C11, S11BH) gene in human (Gene ID 1584). CYP11B1 protein is a member of the cytochrome P450 superfamily of monooxygenases that catalyze reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. It is localized to the inner mitochondrial membrane and mediates the conversion of progesterone to cortisol in the adrenal cortex. CYP11B1 gene mutations are known causes of adrenal hyperplasia 4 (AH4), a form of congenital adrenal hyperplasia due to defective synthesis of cortisol.

Immunogen

Chicken serum albumin-conjugated linear peptide corresponding to human CYP11B1 near the N-terminus.
Epitope: Near N-terminus

Application

Anti-CYP11B1 Antibody, clone 80-7 is an antibody against CYP11B1 for use in Western Blotting and Immunohistochemistry.
Research Category
Signaling
Research Sub Category
Signaling Neuroscience
Western Blotting Analysis: 0.5 µg/mL from a representative lot detected exogenously expressed human CYP11B1, but not CYP11B1 in 10 µg of HEK293 cell lysate.
Western Blotting Analysis: A representative lot detected transfected CYP11B1 in HEK293 cells and endogenous CYP11B1 in mitochondrial preparation from human adrenocortical cell line HAC15 (Gomez-Sanchez, C.E., et al. 2014 Mol. Cell. Endocrinol. 383(1-2):111-117).
Immunohistochemistry Analysis: A representative lot detected CYP11B1 in normal human adrenal tissue.

Quality

Evaluated by Western Blotting in PC12 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected CYP11B1 in 10 µg of PC12 cell lysate.

Target description

~52 kDa observed. Uncharacterized band(s) may appear in some lysates.

Physical form

Format: Purified
Protein G Purified
Purified rat monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Jiao Lu et al.
Cardiovascular research, 114(2), 233-246 (2017-11-11)
High salt intake markedly enhances hypertension induced by angiotensin II (Ang II). We explored central and peripheral slow-pressor mechanisms which may be activated by Ang II and salt. In protocol I, Wistar rats were infused subcutaneously with low-dose Ang II
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EBioMedicine, 103, 105087-105087 (2024-04-04)
The human adrenal cortex consists of three functionally and structurally distinct layers; zona glomerulosa, zona fasciculata (zF), and zona reticularis (zR), and produces adrenal steroid hormones in a layer-specific manner; aldosterone, cortisol, and adrenal androgens, respectively. Cortisol-producing adenomas (CPAs) occur
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Somatic mutations for excess aldosterone production have been frequently identified as important roles in the pathogenesis of unilateral primary hyperaldosteronism (uPA). Although CACNA1H mutation represents a minor etiology in primary aldosteronism, it plays a significant role in causing uPAs in
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Nature medicine, 29(1), 190-202 (2023-01-17)
Primary aldosteronism (PA) due to a unilateral aldosterone-producing adenoma is a common cause of hypertension. This can be cured, or greatly improved, by adrenal surgery. However, the invasive nature of the standard pre-surgical investigation contributes to fewer than 1% of
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