MABE306

Anti-Dnmt1 Antibody, clone DNM-2C1

clone DNM-2C1, from rat

• Synonym(s): DNA (cytosine-5)-methyltransferase 1, Dnmt1, CXXC-type zinc finger protein 9, DNA methyltransferase HsaI, DNA Mtase Hsal, M. Hsal, MCMT
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rat
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: DNM-2C1, monoclonal
• species reactivity: human
• technique(s): ChIP: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• isotype: IgG2b
• NCBI accession no.: NP_001124295
• UniProt accession no.: P26358
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... DNMT1(1786)

Description

General description

DNA (cytosine-5)-methyltransferase 1 (Dnmt1) is one of three mammalian enzymes that catalyze the addition of a methyl group to the 5’ carbon of cytosine residues in CpG dinucleotides. These methylation marks are heritable modifications that are transferred to new DNA molecules following replication, by the activity of Dnmt1, and possibly Dnmt3a and Dnmt3b. Dnmt1 recognizes hemimethylated CpG regions with the aid of the UHRF1 protein, and it mediates the methylation of cytosines at those sites. DNA methylation has been linked to chromosome stability and gene expression in somatic cells; and to parental imprinting and X-chromosome inactivation in germ cells. Several studies have indicated that DNA methylation patterns are altered in various cancers.

Immunogen

His-tagged recombinant protein corresponding to human Dnmt1.

Application

Anti-Dnmt1 Antibody, clone DNM-2C1 is a Rat Monoclonal Antibody for detection of Dnmt1 also known as DNA (cytosine-5)-methyltransferase 1 & has been validated in IP, ChIP & WB.

Immunoprecipitation Analysis: A representative lot was used by an independent laboratory to detect Dnmt1 in IP (Felle, M., et al. (2011). Nucleic Acids Res. 39(19):8355-8365.).

Chromatin Immunoprecipitation Analysis: A representative lot was used by an independent laboratory to immunoprecipitate Dnmt1 in ChIP (Felle, M., et al. (2011). Nucleic Acids Res. 39(19):8355-8365.).

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Chromatin Biology

Quality

Evaluated by Western Blot in HeLa nuclear extract.

Western Blot Analysis: 0.02 µg/mL of this antibody detected Dnmt1 in 10 µg of HeLa nuclear extract.

Target description

~183 kDa observed. Three isoforms are known to exist at ~183 kDa, ~185 kDa, and ~145 kDa An uncharacterized band may be observed at ~90 kDa in some nuclear extracts.

Physical form

Format: Purified

Protein G Purified

Purified rat monoclonal IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
HeLa nuclear extract

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable