IF002
Interferon-γ Protein, Recombinant human
The Interferon-g protein (or IFN-gamma protein) is a lymphoid factor which possesses potent anti-viral activity.
Synonym(s):
Human Interferon, Human Interferon-γ, Recombinant Interferon-γ
Sign Into View Organizational & Contract Pricing
All Photos(1)
About This Item
UNSPSC Code:
12352202
eCl@ss:
32160405
NACRES:
NA.77
Recommended Products
biological source
human
Quality Level
Assay
>98% (SDS-PAGE and HPLC)
specific activity
≥2 x 10(E7) U/mg
manufacturer/tradename
Chemicon®
technique(s)
cell culture | mammalian: suitable
impurities
<0.1 ng/μg endotoxin (of IFN-gamma; 1EU/μg)
input
sample type hematopoietic stem cell(s)
sample type mesenchymal stem cell(s)
NCBI accession no.
UniProt accession no.
shipped in
dry ice
Gene Information
human ... IFNG(3458)
Related Categories
General description
Interferon-g (IFN-gamma) is a lymphoid factor which possesses potent anti-viral activity. It has also been shown to stimulate macrophages and NK cells. Human IFN-gamma is a 16.7 kDa protein containing 143 amino acid residues.
Product Source: Human, expressed in E.coli
Application
For most in vitro applications, IFN-gamma exerts its biological activity in the concentration range of 0.1 to 10 ng/mL. Responding cells are (partial list): Lymphocytes.
Linkage
Replaces: IF3
Physical form
Sterile filtered, lyophilized from 10 mM sodium phosphate, pH 7.4.
Storage and Stability
The lyophilized IFN-g, though stable at room temperature, is best stored desiccated at -20°C. After a quick spin, reconstitute in 1x PBS, pH 8.0 to a concentration of 0.5-1.0 mg/mL. Note: Further dilutions should be made into RPMI + 10% FCS or 5% HSA and stored at 4°C for 1 week or -20°C for future use. Reconstituted material should be stored in undiluted aliquots at -20°C for up to six months.
Analysis Note
Specific Activity: Human IFN-gamma is fully biologically active when compared to standards.The ED(50) as determined in a viral resistance assay is ≤ 0.05 ng/mL
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Nadine Vollmuth et al.
eLife, 11 (2022-09-27)
Chlamydia trachomatis (Ctr) can persist over extended times within their host cell and thereby establish chronic infections. One of the major inducers of chlamydial persistence is interferon-gamma (IFN-γ) released by immune cells as a mechanism of immune defence. IFN-γ activates
Runzhong Fu et al.
Scientific reports, 10(1), 1455-1455 (2020-01-31)
Autoimmune dacryoadenitis and altered lacrimal gland (LG) secretion are features of Sjögren's syndrome (SS). Activity of cathepsin S (CTSS), a cysteine protease, is significantly and specifically increased in SS patient tears. The soluble chemokine, CX3CL1 (fractalkine), is cleaved from membrane-bound
D Frasca et al.
British journal of haematology, 106(2), 317-324 (1999-08-25)
Sublethally irradiated mice were injected with recombinant cytokines to stimulate haemopoietic reconstitution. Interleukin (IL)-11 and IL-6 were able to significantly accelerate the recovery of thymus, spleen and bone marrow cells when used in combination with IL-3, but not alone. Stem
Yan Li et al.
Clinical & translational immunology, 11(12), e1430-e1430 (2022-12-02)
Pancreatic ductal adenocarcinoma (PDAC) is an aggressive disease with a poor prognosis. PDAC has poor response to immunotherapy because of its unique tumour microenvironment (TME). In an attempt to stimulate immunologically silent pancreatic cancer, we investigated the role of epigenetic
Zhijie Chen et al.
Cell chemical biology, 28(11), 1542-1553 (2021-02-14)
Peroxynitrite is a reactive nitrogen species (RNS) that plays critical roles in signal transduction, stress response, and numerous human diseases. Advanced molecular tools that permit the selective, sensitive, and noninvasive detection of peroxynitrite are essential for understanding its pathophysiological functions.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service