ABE1425
Anti-COUP-TF1 Antibody
serum, from rabbit
Synonym(s):
COUP transcription factor 1, COUP-TF1, COUP transcription factor I, COUP-TF I, Nuclear receptor subfamily 2 group F member 1, V-erbA-related protein 3, EAR-3, COUP-TF1
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All Photos(3)
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
serum
antibody product type
primary antibodies
clone
polyclonal
species reactivity
mouse, human, rat
technique(s)
ChIP: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
mouse ... Nr2F1(13865)
rat ... Nr2F1(81808)
General description
COUP transcription factor 1 (UniProt Q60632; also known as Avian erythroblastic leukemia viral oncogene homolog-like 3, COUP-TF1, COUP-TF I, COUP transcription factor I, EAR-3, Nuclear receptor subfamily 2 group F member 1, V-erbA-related protein 3) is encoded by the Nr2f1 (also known as Erbal3, Tfcoup1) gene (Gene ID 13865) in murine species. COUP transcription factors1&2 (COUP-TFI & COUP-TFII) are two related nuclear orphan receptors implicated in their roles in guiding migrating neurons towards their target regions during brain development. COUP-TFI and COUP-TFII are expressed in distinct and non-overlapping migrating cells in specific pathways in the basal telencephalon of mouse embryos at a stage when interneuron migration is robust. While COUP-TFI appears to control dorsal and ventral migration in the basal telencephalon, COUP-TFII is more specific in modulating tangential migration into the intermediate zone of the cortex. Coup-TFII is also identified as an activator of Prox1 expression in venous lymphatic endothelial cell (LEC) progenitors during early stages of LEC specification and differentiation.
Specificity
Specificity of the antiserum was confirmed by immunogen blocking in immunohistochemistry on frozen sections (Tripodi, M., et al. (2004). Development. 131(24):6119-6129).
Immunogen
Epitope: N-terminal half
GST-tagged recombinant protein corresponding to the N-terminal half of mouse COUP-TF1.
Application
Anti-COUP-TF1 Antibody is an antibody against COUP-TF1 for use in Western Blotting, Immunohistochemistry, Immunofluorescence, Chromatin Immunoprecipitation (ChIP).
Immunohistochemistry Analysis: An 1:250 dilution from a representative lot detected COUP-TFI in 20 µg of rat cerebral cortex, human cerebellum, and human small intestine tissue.
Immunofluorescence Analysis: An 1:500-1000 dilution of a representative lot detected COUP-TF1 immunoreactivity in the developing brain using paraformaldehyde-fixed cryosections from wild-type mouse E13.5 embryos, but not in embryos with brain region-specific COUP-TF1-knockout, by fluorescent immunohistochemistry (Courtesy of Dr. Michele Studer, Institut de Biologie Valrose, France).
Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected COUP-TF1 occupancy at the conserved binding sites along the Rnd2 locus using chromatin preparations from the neocortices of E14.5 mouse embryos (Alfano, C., et al. (2011). Development. 138(21):4685-4697).
Western Blotting Analysis: A representative lot detected a higher COUP-TF1 level in retinal and retinal pigment epithelium (RPE) extracts from 24-week-old mice subjected to prorolonged light exposure (24 h/day) than from mice with normal light exposure (12 h/day). Light stress-induced COUP-TF1 upregulation was also observed in RPE, but not retinal extracts from Ranbp2+/− mice (Cho, K.I., et al. (2010). Dis. Model Mech. 3(9-10):595-604).
Western Blotting Analysis: A representative lot detected a 64 kDa form of COUP-TF1 co-immunoprecipitated with RNABP2 from murine retinal extracts (Cho, K.I., et al. (2010). Dis. Model Mech. 3(9-10):595-604).
Immunofluorescence Analysis: A representative lot detected cortex-specific COUP-TFI-knockout in the developing brain of E11.5 COUP-TFIfl/flEmx1Cre transgenic mouse embryos by fluorescent immunohistochemistry staining of paraformaldehyde-fixed cryosections (Armentano, M., et al. (2007). Nat. Neurosci. 10(10):1277-1286).
Immunofluorescence Analysis: A representative lot detected A representative lot detected COUP-TFI immunoreactivity in paraformaldehyde-fixed frozen coronal sections from wild-type E15.5 mouse embryos, but not from COUP-TFI-knockout embryos (Armentano, M., et al. (2006). Development. 133(21):4151-4162).
Immunofluorescence Analysis: A representative lot detected COUP-TF1 immunoreactivity associated with calbindin-positive GABAergic interneurons, but not reelin-positive Cajal-Retzius cells in the developing cortex by dual fluorescent immunohistochemistry using cryosections from E13.5 and E15.5 mouse embryos (Tripodi, M., et al. (2004). Development. 131(24):6119-6129).
Immunohistochemistry Analysis: A representative lot detected a different COUP-TF1 expression pattern than that of COUP-TF2 in the developing telencephalon of forebrain using cryosections from E13.5 mouse embryos (Tripodi, M., et al. (2004). Development. 131(24):6119-6129).
Immunofluorescence Analysis: An 1:500-1000 dilution of a representative lot detected COUP-TF1 immunoreactivity in the developing brain using paraformaldehyde-fixed cryosections from wild-type mouse E13.5 embryos, but not in embryos with brain region-specific COUP-TF1-knockout, by fluorescent immunohistochemistry (Courtesy of Dr. Michele Studer, Institut de Biologie Valrose, France).
Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected COUP-TF1 occupancy at the conserved binding sites along the Rnd2 locus using chromatin preparations from the neocortices of E14.5 mouse embryos (Alfano, C., et al. (2011). Development. 138(21):4685-4697).
Western Blotting Analysis: A representative lot detected a higher COUP-TF1 level in retinal and retinal pigment epithelium (RPE) extracts from 24-week-old mice subjected to prorolonged light exposure (24 h/day) than from mice with normal light exposure (12 h/day). Light stress-induced COUP-TF1 upregulation was also observed in RPE, but not retinal extracts from Ranbp2+/− mice (Cho, K.I., et al. (2010). Dis. Model Mech. 3(9-10):595-604).
Western Blotting Analysis: A representative lot detected a 64 kDa form of COUP-TF1 co-immunoprecipitated with RNABP2 from murine retinal extracts (Cho, K.I., et al. (2010). Dis. Model Mech. 3(9-10):595-604).
Immunofluorescence Analysis: A representative lot detected cortex-specific COUP-TFI-knockout in the developing brain of E11.5 COUP-TFIfl/flEmx1Cre transgenic mouse embryos by fluorescent immunohistochemistry staining of paraformaldehyde-fixed cryosections (Armentano, M., et al. (2007). Nat. Neurosci. 10(10):1277-1286).
Immunofluorescence Analysis: A representative lot detected A representative lot detected COUP-TFI immunoreactivity in paraformaldehyde-fixed frozen coronal sections from wild-type E15.5 mouse embryos, but not from COUP-TFI-knockout embryos (Armentano, M., et al. (2006). Development. 133(21):4151-4162).
Immunofluorescence Analysis: A representative lot detected COUP-TF1 immunoreactivity associated with calbindin-positive GABAergic interneurons, but not reelin-positive Cajal-Retzius cells in the developing cortex by dual fluorescent immunohistochemistry using cryosections from E13.5 and E15.5 mouse embryos (Tripodi, M., et al. (2004). Development. 131(24):6119-6129).
Immunohistochemistry Analysis: A representative lot detected a different COUP-TF1 expression pattern than that of COUP-TF2 in the developing telencephalon of forebrain using cryosections from E13.5 mouse embryos (Tripodi, M., et al. (2004). Development. 131(24):6119-6129).
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Nuclear Receptors
Nuclear Receptors
Quality
Evaluated by Western Blotting in mouse brain tissue lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected COUP-TFI in 20 µg of mouse brain tissue lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected COUP-TFI in 20 µg of mouse brain tissue lysate.
Target description
~50-55kDa kDa observed. Uncharacterized band(s) may appear in some lysates.
Physical form
Rabbit polyclonal antibody in serum with 0.05% sodium azide.
Unpurified
Storage and Stability
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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