71194-M
0.5 M THP Solution
Reducing agent compatible with His•Bind chromatography
Synonym(s):
Tris(3-hydroxypropyl)phosphine
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About This Item
UNSPSC Code:
41106511
NACRES:
NA.77
Recommended Products
Quality Level
form
solution
manufacturer/tradename
Novagen®
storage condition
OK to freeze
technique(s)
protein purification: suitable
application(s)
sample preparation
storage temp.
−20°C
Related Categories
General description
Reducing agent compatible with HIS-BIND® chromatography
THP [Tris(hydroxypropyl)phosphine; C9H21O3P] is a water-soluble, odorless, sulfhydryl reducing agent that is more stable and effective than dithiothreitol (DTT). Against oxidized glutathione, the reducing capacity of THP (0.232 meq/g) is greater than that of TCEP (0.182 meq/g). THP is also more resistant to air oxidation than DTT and is compatible with immobilized metal affinity chromatography with HIS-BIND® and Ni-NTA His•Bind resins at concentrations up to 1.0 mM. The common working concentration of THP for many applications is 15 mM. THP is provided as a ready-to-use 0.5 M solution in water.
Purity: >80% by NMR. CAS: 4706-17-6. MW: 208.2.
Purity: >80% by NMR. CAS: 4706-17-6. MW: 208.2.
Features and Benefits
Compatability with common methods for purifying His-tagged proteins - immobilized metal affinity chromatography with HIS-BIND® and Ni-NTA His•Bind resins at concentrations up to 1.0 mM
Stability: More stable and effective than traditional reducing agents like dithiothreitol (DTT)
Reducing Capacity: greater reducing capacity than TCEP against oxidized glutathione
Ready-to-use: THP is provided as a ready-to-use 0.5 M solution in water, making it easy to use in many applications with flexible usage
Stability: More stable and effective than traditional reducing agents like dithiothreitol (DTT)
Reducing Capacity: greater reducing capacity than TCEP against oxidized glutathione
Ready-to-use: THP is provided as a ready-to-use 0.5 M solution in water, making it easy to use in many applications with flexible usage
Warning
Toxicity: Corrosive (L)
Other Notes
Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
Legal Information
HIS-BIND is a registered trademark of Merck KGaA, Darmstadt, Germany
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Dam. 1 - Skin Corr. 1B
Storage Class Code
8B - Non-combustible corrosive hazardous materials
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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