Note: 1 T = 1 test. Sufficient for 10 Western miniblots.
Rabbit polyclonal antibody adsorbed against non-phosphopeptide corresponding to the immunogen phophorylation site, followed by immunoaffinity chromatography. Recognizes the ~65-68 kDa PKR protein phosphorylated at Thr451.
Recognizes the ~65-68 kDa PKR protein phosphorylated at Thr451.
This PhosphoDetect Anti-PKR (pThr⁴⁵¹) Rabbit pAb is validated for use in Immunoblotting, Immunohistochemistry for the detection of PKR (pThr⁴⁵¹).
Immunogen
a synthetic phosphopeptide corresponding to amino acids surrounding the Thr⁴⁵¹ phosphorylation site of human PKR (double-stranded RNA-dependent protein kinase)
Application
Immunoblotting (1:1000)
Immunohistochemistry (see comments)
Warning
Toxicity: Standard Handling (A)
Physical form
In Dulbecco′s PBS (without Mg+2 or Ca+2), 0.1% BSA, 50% glycerol, pH 7.3.
Reconstitution
Following initial thaw, aliquot and freeze (-70°C).
Analysis Note
Positive Control IFNγ-stimulated HeLa cells.
Other Notes
Cuddihy, A.R., et al.1999. Oncogene8, 2690. Srivastava, S.P., et al. 1998. J. Biol. Chem.273, 2416. Clemens, M.J. 1997. Int. J. Biochem. Cell Biol.29, 945.
When used at a 1:1000 dilution there is sufficient antibody for 10 blots at 10 ml/blot. This antibody has also been reported to work for immunohistochemistry. Variables associated with assay conditions will dictate the optimal dilution.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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