A 10 mM solution of ALLN (Cat. No. 208719) in anhydrous DMSO. Inhibitor of calpain I (Ki = 190 nM), calpain II (Ki = 220 nM), cathepsin B (Ki = 150 nM), and cathepsin L (Ki = 0.5 nM). Inhibits neutral cysteine proteases and proteasome (Ki = 6 µM). Protects against neuronal damage caused by hypoxia and ischemia. Inhibits apoptosis in thymocytes and metamyelocytes. Inhibits cell cycle progression at the G1/S border and metaphase/anaphase in CHO cells by inhibiting cyclin B degradation. Also prevents nitric oxide production by activated macrophages by interfering with transcription of the inducible nitric oxide synthase gene. Inhibits the proteolysis of IkB-α and IkB-β by the ubiquitin-proteasome complex.
Biochem/physiol Actions
Cell permeable: yes
Primary Target calpain 1
Product does not compete with ATP.
Reversible: no
Target Ki: 190 nM, 220 nM, 150 nM, 500 pM, against calpain I, calpain II, cathepsin B, and cathepsin L, respectively
Packaging
Packaged under inert gas
Warning
Toxicity: Flammable (J)
Physical form
A 10 mM (5 mg/1.30 ml) solution of ALLN (Cat. No. 208719) in DMSO.
Reconstitution
Following initial thaw, aliquot and freeze (-20°C).
Other Notes
Milligan, S.A., et al. 1996. Arch. Biochem. Biophys.335, 388. Griscavage, J.M., et al. 1995. Biochem. Biophys. Res. Commun.215, 721. Squier, M.K., et al. 1994. J. Cell Physiol.159, 229. Rami, J., and Kreiglstein, J. 1993. Brain Res.609, 67.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point(F)
188.6 °F - closed cup
Flash Point(C)
87 °C - closed cup
Certificates of Analysis (COA)
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