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176002

Sigma-Aldrich

Anti-Angiopoietin-2 Rabbit pAb

liquid, Calbiochem®

Synonym(s):

Anti-Ang-2

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.43

biological source

rabbit

Quality Level

100

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

liquid

does not contain

preservative

species reactivity

mouse

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

isotype

IgG

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

General description

Anti-Angiopoietin-2, rabbit polyclonal, recognizes the ~55 kDa non-glycosylated and the ~70 kDa glycosylated, secreted Ang-2 protein. It is validated for ELISA, WB, IF, and IHC.
Immunoaffinity purified rabbit polyclonal antibody. Recognizes both the ~55 kDa nonglycosylated and the ~70 kDa glycosylated forms of angiopoietin-2.
Recognizes both the ~55 kDa non-glycosylated and the ~70 kDa glycosylated, secreted Ang-2 protein in cell culture medium. Does not cross-react with Ang-1.
  • Antibody Target Gene Symbol: ANGPT2
  • Target Synonym: AGPT2, ANG II, ANG-2, ANGIOPOIETIN, ANGIOPOIETIN 2, Angiotensin 2, Angrp
  • Entrez Gene Name: angiopoietin 2
  • Hu Entrez ID: 285
  • Mu Entrez ID: 11601
  • Rat Entrez ID: 89805

    • Immunogen

    a synthetic peptide corresponding to a 21 amino acid cytoplasmic sequence at the N-terminus of mouse angiopoietin-2

    Application

    ELISA (0.1-1 µg/ml)

    Immunoblotting (1-10 µg/ml)

    Immunofluorescence (2-20 µg/ml)

    Immunohistochemistry (2-20 µg/ml)

    Packaging

    Please refer to vial label for lot-specific concentration.

    Warning

    Toxicity: Standard Handling (A)

    Other Notes

    Maisonpierre, P.C., et al. 1997. Science 277, 55.
    Davis, S., et al. 1996. Cell 87, 1161.
    Suri, S. et al. 1996. Cell 87, 1171.
    The immunogenic peptide sequence is 71% (15/21 amino acids) conserved in the human angiopoietin-2 protein. Does not cross-react with angiopoietin-1. Variables associated with assay conditions will dictate the optimal working dilution.

    Legal Information

    CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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    Storage Class Code

    10 - Combustible liquids

    WGK

    WGK 1

    Flash Point(F)

    Not applicable

    Flash Point(C)

    Not applicable

    Certificates of Analysis (COA)

    Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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    Earlier studies have reported that transforming growth factor beta 1(TGFβ1) is a critical mediator of hyperoxia-induced acute lung injury (HALI) in developing lungs, leading to impaired alveolarization and a pulmonary phenotype of bronchopulmonary dysplasia (BPD). However, the mechanisms responsible for

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